Document Detail


Interleukin-8 levels and granulocyte counts in cervical mucus during pregnancy.
MedLine Citation:
PMID:  10735598     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PROBLEM: We measured interleukin-8 (IL-8) and granulocyte counts in cervical mucus to assess local immunity and parturition. METHOD OF STUDY: We detected IL-8 by enzyme linked immunosorbent assay (ELISA) and Western blotting. Granulocytes were counted on a slide glass containing mucus from the external cervical os. RESULTS: ELISA and Western blotting revealed IL-8 in cervical mucus from both nonpregnant and pregnant women. There were no significant differences in cervical mucus IL-8 levels or granulocyte counts between follicular, ovulatory, and luteal phases. However, IL-8 and granulocyte counts were significantly decreased after menopause. IL-8 and granulocyte counts were increased significantly during pregnancy, and were further increased after 38 weeks of gestation and at labor. IL-8 levels were significantly correlated with granulocyte counts, based on the study of 678 samples (P <0.0001). CONCLUSION: IL-8 is involved in the increase of cervical granulocytes and in the process of parturition.
Authors:
L Luo; T Ibaragi; M Maeda; M Nozawa; T Kasahara; M Sakai; Y Sasaki; K Tanebe; S Saito
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  43     ISSN:  1046-7408     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-06-05     Completed Date:  2000-06-05     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  78-84     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Beijing Hospital, China.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Blotting, Western
Cervix Mucus / cytology,  immunology*
Enzyme-Linked Immunosorbent Assay
Female
Granulocytes / cytology,  immunology,  metabolism*
Humans
Interleukin-8 / metabolism*
Leukocyte Count
Middle Aged
Postpartum Period / metabolism
Pregnancy
Chemical
Reg. No./Substance:
0/Interleukin-8

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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