Document Detail

Interleukin-8 increases acetylcholine response of rat intestinal segments.
MedLine Citation:
PMID:  11472327     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Interleukin-8 (IL-8) is a pro-inflammatory cytokine highly expressed in inflammatory bowel diseases, but whose effects on intestinal motility are unknown. AIM: To characterize the role of IL-8 in the contraction of rat intestinal segments. METHODS: Contractile response to acetylcholine (ACh 10-6 M) in terminal ileal segments (including mucosa) from Wistar rats was measured before and after incubation (15, 30, 60 or 90 min) with IL-8 (1 ng/mL), and after 60 min of incubation with different doses of IL-8 (0, 0.1, 0.5, 1, 10 and 100 ng/mL). The effects of blocking neural transmission with tetrodotoxin (TTX) and inhibiting protein synthesis (cycloheximide) were tested. The contractile response of longitudinal muscle-myenteric plexus preparations (i.e. without mucosa) was measured after 60 min of incubation with 0.1 and 1 ng/mL of IL-8. RESULTS: IL-8 increased ileal contraction induced by ACh 10(-6) M. This augmentation was significant after 60 min of incubation (58%, P=0.01) and persisted after 90 min (18%, P=0.04). A 60-min incubation period showed a dose-related effect, beginning at 0.5 ng/mL (30%, P=0.003) and reaching a peak at 1 ng/mL (58%, P=0.01). The same effect was also observed on colonic segments. TTX did not affect the IL-8 increase of ACh-induced contractions, which was completely abolished by cycloheximide. IL-8 had no significant effect on longitudinal muscle-myenteric plexus preparations. CONCLUSION: In vitro, IL-8 increases contractile response of the ileum to ACh in a dose-dependent manner. This effect is not neurally mediated, but seems to involve protein synthesis by intestinal mucosa.
V Plattner; V Leray; M D Leclair; A C Aubé; C Cherbut; J P Galmiche
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Alimentary pharmacology & therapeutics     Volume:  15     ISSN:  0269-2813     ISO Abbreviation:  Aliment. Pharmacol. Ther.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-07-26     Completed Date:  2001-12-19     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8707234     Medline TA:  Aliment Pharmacol Ther     Country:  England    
Other Details:
Languages:  eng     Pagination:  1227-32     Citation Subset:  IM    
Human Nutrition Research Center, INSERM U 539, Hôtel-Dieu, Nantes, France.
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MeSH Terms
Acetylcholine / pharmacology*
Cycloheximide / pharmacology
Gastrointestinal Motility / physiology
Interleukin-8 / administration & dosage,  physiology*
Intestines / drug effects*,  metabolism
Muscle Contraction / drug effects
Muscle, Smooth / drug effects
Protein Synthesis Inhibitors / pharmacology
Rats, Wistar
Tetrodotoxin / pharmacology
Vasodilator Agents / pharmacology*
Reg. No./Substance:
0/Interleukin-8; 0/Protein Synthesis Inhibitors; 0/Vasodilator Agents; 4368-28-9/Tetrodotoxin; 51-84-3/Acetylcholine; 66-81-9/Cycloheximide

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