| Interleukin-7 modulates extracellular matrix production and TGF-beta signaling in cultured human subconjunctival fibroblasts. | |
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MedLine Citation:
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PMID: 16769608 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: We examined the role of interleukin-7 (IL-7) in modulation of production of extracellular matrix (ECM), immunolocalization of Smads, and cell migration and expressions of transforming growth factor-beta (TGF-beta) in cultured human subconjunctival fibroblasts. IL-7 is capable of inducing Smad7, an inhibitory Smad that interferes with TGF-beta/Smad signal. METHODS: The effects of IL-7 on ECM production, immunolocalization of Smads, type I collagen, fibronectin, alpha -smooth muscle actin (alpha -SMA), and cell migration were examined in human subconjunctival fibroblast culture with or without TGF-beta1. ECM production, such as type I collagen and fibronectin, was measured by immunoassay or real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Cell migration was examined using an in vitro wound model in monolayer cultures. We also examined the effects of IL-7, PKC inhibitor, and STAT inhibitor on the expressions of TGF-beta1 and type I collagen alpha1 chain (col1A1) m-RNA by using real-time RT-PCR. RESULTS: IL-7 reduced the ECM production much more markedly in the cells treated with TGF-1beta than in the control fibroblasts. TGF-beta1 strongly showed immunolocalization of phospho-Smad2, and IL-7 also showed immunolocalization of Smad7 in the nuclei. The immunoreactivities of alpha -SMA and fibronectin were weaker in the presence of IL-7 than in the control cells. IL-7 also delayed defect closure in the monolayer cell sheets, and the delay was recovered by exogenous type I collagen or fibronectin. Each of IL-7, BIS I, or AGS 490 reduced the mRNA expressions of TGF-beta1 and col1A1. CONCLUSIONS: These findings indicate that IL-7 is involved in ECM production in the subconjunctival fibroblasts activated by exogenous TGF-beta1, suggesting that administration of IL-7 can be a novel therapeutic strategy in preventing undesirable bleb scar formation during healing after filtration surgery. |
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Authors:
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Osamu Yamanaka; Shizuya Saika; Kazuo Ikeda; Ken-Ichi Miyazaki; Yoshitaka Ohnishi; Akira Ooshima |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current eye research Volume: 31 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-13 Completed Date: 2006-08-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 491-9 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan. yamanaka@wakayama-med.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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genetics Cell Movement / drug effects Cells, Cultured Collagen Type I / genetics Conjunctiva / cytology, drug effects*, metabolism Extracellular Matrix / metabolism* Extracellular Matrix Proteins / biosynthesis* Fibroblasts / drug effects, metabolism Fibronectins / genetics Humans Interleukin-7 / pharmacology* RNA, Messenger / biosynthesis Reverse Transcriptase Polymerase Chain Reaction Signal Transduction* Smad Proteins / metabolism Transforming Growth Factor beta / genetics, metabolism*, pharmacology Transforming Growth Factor beta1 Wound Healing / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Collagen Type I; 0/Extracellular Matrix Proteins; 0/Fibronectins; 0/Interleukin-7; 0/RNA, Messenger; 0/Smad Proteins; 0/TGFB1 protein, human; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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