Document Detail


Interleukin-7 activates p56lck and p59fyn, two tyrosine kinases associated with the p90 interleukin-7 receptor in primary human T cells.
MedLine Citation:
PMID:  7589097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have investigated signaling events associated with the cloned 90-kDa (p90) interleukin-7 receptor (IL-7R) to determine whether changes in the signaling pathways initiated by this molecule can explain the ability of T cells to proliferate to IL-7 following activation. Using in vitro kinase assays we find that the p90 IL-7R in both unstimulated and activated human T cells is physically associated with two molecules with intrinsic kinase activity. Western blotting analysis reveals these proteins to be the src kinase enzymes, p59fyn and p56lck. Binding of human recombinant IL-7 to the p90 IL-7R results in increased activity of both receptor-associated kinases in both resting and activated mature T cells. Thus, the signaling pathways initiated via the p90 IL-7R-associated src kinases are unlikely to be solely responsible for the proliferation of only activated T cells in response to IL-7. Additional signals, which may derive from other IL-7R-associated molecules such as the gamma c, are clearly required for IL-7-driven proliferation of activated primary T cells.
Authors:
T H Page; F V Lali; B M Foxwell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  25     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1995-12-12     Completed Date:  1995-12-12     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  2956-60     Citation Subset:  IM    
Affiliation:
Kennedy Institute of Rheumatology, London, GB.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / metabolism*
Cell Division / drug effects
Cells, Cultured
Cloning, Molecular
Enzyme Activation / drug effects
Humans
Interleukin-7 / pharmacology*
Lymphocyte Activation*
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Macromolecular Substances
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins c-fyn
Receptors, Interleukin / metabolism*
Receptors, Interleukin-7
Recombinant Proteins / pharmacology
Signal Transduction / physiology*
T-Lymphocytes / drug effects*,  enzymology
src-Family Kinases / metabolism*
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Interleukin-7; 0/Macromolecular Substances; 0/Proto-Oncogene Proteins; 0/Receptors, Interleukin; 0/Receptors, Interleukin-7; 0/Recombinant Proteins; EC 2.7.10.2/FYN protein, human; EC 2.7.10.2/Lymphocyte Specific Protein Tyrosine Kinase p56(lck); EC 2.7.10.2/Proto-Oncogene Proteins c-fyn; EC 2.7.10.2/src-Family Kinases

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