| Interleukin-6 receptor expression in contracting human skeletal muscle: regulating role of IL-6. | |
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MedLine Citation:
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PMID: 15837717 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Contracting muscle fibers produce and release IL-6, and plasma levels of this cytokine are markedly elevated in response to physical exercise. We recently showed autocrine regulation of IL-6 in human skeletal muscle in vivo and hypothesized that this may involve up-regulation of the IL-6 receptor. Therefore, we investigated IL-6 receptor regulation in response to exercise and IL-6 infusion in humans. Furthermore, using IL-6-deficient mice, we investigated the role of IL-6 in the IL-6 receptor response to exercise. Human skeletal muscle biopsies were obtained in relation to: 3 h of bicycle exercise and rest (n=6+5), or recombinant human IL-6 infusion (rhIL-6) or saline infusion (n=6+6). We further obtained skeletal muscle samples from IL-6 knockout (KO) mice and wild-type C57/BL-6 mice in response to a 1-h bout of exercise. In exercising human skeletal muscle, IL-6 receptor mRNA increased sixfold with a peak at 6 h postexercise. Detection of the IL-6 receptor protein by immunohistochemistry revealed a pronounced staining following exercise that was primarily located at the cell membrane of the muscle fibers, whereas muscle gp130 expression and plasma levels of soluble IL-6 receptor were unaffected. Infusion of rhIL-6 to humans had no effect on the mRNA level of the IL-6 receptor, whereas there was an increase at the protein level. IL-6 receptor mRNA increased similarly in muscle of both IL-6 KO mice and wild-type mice in response to exercise. In conclusion, exercise increases IL-6 receptor production in human skeletal muscle. This effect is most prominent 6 h after the end of the exercise bout, suggesting a postexercise-sensitizing mechanism to IL-6 when plasma IL-6 is concomitantly low. Exercise-induced increases in IL-6 receptor mRNA most likely occurs via an IL-6 independent mechanism as shown in IL-6 KO mice and the human rhIL-6 infusion study, whereas IL-6 receptor protein levels are responsive to elevated plasma IL-6 levels. |
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Authors:
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Pernille Keller; Milena Penkowa; Charlotte Keller; Adam Steensberg; Christian P Fischer; Mercedes Giralt; Juan Hidalgo; Bente Klarlund Pedersen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-04-18 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 19 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-06-29 Completed Date: 2006-02-23 Revised Date: 2012-02-15 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 1181-3 Citation Subset: IM |
Affiliation:
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Centre of Inflammation and Metabolism, Department of Infectious Diseases, University Hospital of Copenhagen, Copenhagen, Denmark. pernillekeller@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Cytokine Receptor gp130 / physiology Exercise* Female Humans Interleukin-6 / physiology* Male Mice Mice, Inbred C57BL Mice, Knockout Muscle, Skeletal / metabolism* RNA, Messenger / analysis Receptors, Interleukin-6 / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-6; 0/RNA, Messenger; 0/Receptors, Interleukin-6; 133483-10-0/Cytokine Receptor gp130 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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