Document Detail

Interleukin-6 receptor expression in contracting human skeletal muscle: regulating role of IL-6.
MedLine Citation:
PMID:  15837717     Owner:  NLM     Status:  MEDLINE    
Contracting muscle fibers produce and release IL-6, and plasma levels of this cytokine are markedly elevated in response to physical exercise. We recently showed autocrine regulation of IL-6 in human skeletal muscle in vivo and hypothesized that this may involve up-regulation of the IL-6 receptor. Therefore, we investigated IL-6 receptor regulation in response to exercise and IL-6 infusion in humans. Furthermore, using IL-6-deficient mice, we investigated the role of IL-6 in the IL-6 receptor response to exercise. Human skeletal muscle biopsies were obtained in relation to: 3 h of bicycle exercise and rest (n=6+5), or recombinant human IL-6 infusion (rhIL-6) or saline infusion (n=6+6). We further obtained skeletal muscle samples from IL-6 knockout (KO) mice and wild-type C57/BL-6 mice in response to a 1-h bout of exercise. In exercising human skeletal muscle, IL-6 receptor mRNA increased sixfold with a peak at 6 h postexercise. Detection of the IL-6 receptor protein by immunohistochemistry revealed a pronounced staining following exercise that was primarily located at the cell membrane of the muscle fibers, whereas muscle gp130 expression and plasma levels of soluble IL-6 receptor were unaffected. Infusion of rhIL-6 to humans had no effect on the mRNA level of the IL-6 receptor, whereas there was an increase at the protein level. IL-6 receptor mRNA increased similarly in muscle of both IL-6 KO mice and wild-type mice in response to exercise. In conclusion, exercise increases IL-6 receptor production in human skeletal muscle. This effect is most prominent 6 h after the end of the exercise bout, suggesting a postexercise-sensitizing mechanism to IL-6 when plasma IL-6 is concomitantly low. Exercise-induced increases in IL-6 receptor mRNA most likely occurs via an IL-6 independent mechanism as shown in IL-6 KO mice and the human rhIL-6 infusion study, whereas IL-6 receptor protein levels are responsive to elevated plasma IL-6 levels.
Pernille Keller; Milena Penkowa; Charlotte Keller; Adam Steensberg; Christian P Fischer; Mercedes Giralt; Juan Hidalgo; Bente Klarlund Pedersen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Retracted Publication     Date:  2005-04-18
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  19     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-29     Completed Date:  2006-02-23     Revised Date:  2014-04-16    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1181-3     Citation Subset:  IM    
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MeSH Terms
Cytokine Receptor gp130 / physiology
Interleukin-6 / physiology*
Mice, Inbred C57BL
Mice, Knockout
Muscle, Skeletal / metabolism*
RNA, Messenger / analysis
Receptors, Interleukin-6 / genetics*
Reg. No./Substance:
0/Interleukin-6; 0/RNA, Messenger; 0/Receptors, Interleukin-6; 133483-10-0/Cytokine Receptor gp130
Retraction In:
FASEB J. 2014 Mar;28(3):1526   [PMID:  24716228 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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