| Interleukin-6 protects human macrophages from cellular cholesterol accumulation and attenuates the pro-inflammatory response. | |
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MedLine Citation:
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PMID: 21757719 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Cholesterol-laden monocyte-derived macrophages are phagocytic cells characteristic of early and advanced atherosclerotic lesions. Interleukin-6 (IL-6) is a macrophage secretory product which is abundantly expressed in atherosclerotic plaques but whose precise role in atherogenesis is unclear. The capacity of macrophages to clear apoptotic cells, through the efferocytosis mechanism, as well as to reduce cellular cholesterol accumulation contributes to prevent plaque progression and instability. By virtue of its capacity to promote cellular cholesterol efflux from phagocyte-macrophages, ABCA1 was reported to reduce atherosclerosis. We demonstrated that lipid loading in human macrophages was accompanied by a strong increase of IL-6 secretion. Interestingly, IL-6 markedly induced ABCA1 expression and enhanced ABCA1-mediated cholesterol efflux from human macrophages to apoAI. Stimulation of ABCA1-mediated cholesterol efflux by IL-6 was however abolished by selective inhibition of the Jak-2/Stat3 signalling pathway. In addition, IL-6 enhanced the capacity of human macrophages to phagocytose apoptotic cells and stimulates the ABCA1-mediated efflux of cholesterol derived from the ingestion of free cholesterol-loaded apoptotic macrophages. Finally, the treatment of human macrophages with IL-6 led to the establishment of an anti-inflammatory cytokine profile, characterized by an increased secretion of IL-4 and IL-10, together with a decrease of that of IL-1b. Taken together, our results suggest that high amounts of IL-6 secreted by lipid laden human macrophages may constitute a protective response from macrophages to prevent accumulation of cytotoxic free cholesterol. Such a cellular recycling of free cholesterol may contribute to reduce both foam cell formation and the accumulation of apoptotic bodies as well as intraplaque inflammation in atherosclerotic lesions. |
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Authors:
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Eric Frisdal; Philippe Lesnik; Maryline Olivier; Paul Robillard; M John Chapman; Thierry Huby; Maryse Guerin; Wilfried Le Goff |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-8 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: - ISSN: 1083-351X ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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INSERM UMR S939, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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