| Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery. | |
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MedLine Citation:
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PMID: 15901266 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Intrauterine infection and inflammation in women with preterm labor are related to adverse perinatal outcome. Due to its subclinical nature, a correct diagnosis depends on retrieval of amniotic fluid. Amniocentesis is, however, not performed as a clinical routine because of its invasiveness. Hypothetically, cytokines in the cervical fluid may represent an alternative diagnostic approach. The aim was to examine cervical interleukin (IL)-6 and IL-8 in relation to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm birth in women in preterm labor. METHODS: Women with singleton pregnancies in preterm labor (<34 weeks of gestation) and intact membranes were included. Cervical (n = 91) and amniotic fluids (n = 56) were collected. Polymerase chain reaction for Ureaplasma urealyticum and Mycoplasma hominis and culture for aerobic and anaerobic bacteria were performed. IL-6 and IL-8 were analyzed with enzyme-linked immunosorbent assay. RESULTS: Non-lactobacillus-dominated biota was detected in cervical secretion in 25% (22/89) and the presence of micro-organisms in the amniotic fluid in 16% (9/56) of the patients. The presence of U. urealyticum in the cervical fluid (21/46) was associated with significantly higher levels of IL-6 in the secretion. IL-6 and IL-8 were significantly higher in cervical fluid of women with intra-amniotic infection and inflammation and in women who delivered < or =7 days and/or before 34 weeks of gestation. Cervical IL-6 > or = 1.7 ng/ml was related to intra-amniotic inflammation (relative risk: 2.67; range: 1.50-4.74) and had a sensitivity, specificity, positive predictive value, and negative predictive value of 58, 83, 75, and 69%, respectively, in the identification of intra-amniotic inflammation. Similar data were obtained for IL-8 > or = 6.7 ng/ml. CONCLUSIONS: High levels of cervical IL-6 and IL-8 are moderately predictive of intrauterine infection/inflammation and preterm delivery. |
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Authors:
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Rose-Marie Holst; Inger Mattsby-Baltzer; Ulla-Britt Wennerholm; Henrik Hagberg; Bo Jacobsson |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta obstetricia et gynecologica Scandinavica Volume: 84 ISSN: 0001-6349 ISO Abbreviation: Acta Obstet Gynecol Scand Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-05-19 Completed Date: 2005-06-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370343 Medline TA: Acta Obstet Gynecol Scand Country: Denmark |
Other Details:
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Languages: eng Pagination: 551-7 Citation Subset: IM |
Affiliation:
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Perinatal Center, Department of Obstetrics and Gynecology, Institute for the Health of Women and Children, Sahlgrenska University Hospital, Göteborg, Sweden. rose-marie.holst@vgregion.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Amniotic Fluid / metabolism, microbiology Biological Markers / metabolism Cervix Uteri / metabolism*, microbiology* Cohort Studies DNA, Bacterial / analysis Enzyme-Linked Immunosorbent Assay Female Humans Interleukin-6 / metabolism Interleukin-8 / metabolism Mycoplasma hominis / genetics, isolation & purification Obstetric Labor, Premature / diagnosis*, metabolism, microbiology Polymerase Chain Reaction Predictive Value of Tests Pregnancy Prenatal Diagnosis / methods Prospective Studies Sensitivity and Specificity Sweden Ureaplasma urealyticum / genetics, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/DNA, Bacterial; 0/Interleukin-6; 0/Interleukin-8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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