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Interleukin-6 Inhibits Regulatory T Cells and Improves the Proliferation and Cytotoxic Activity of Cytokine-induced Killer Cells.
MedLine Citation:
PMID:  22495391     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The presence of regulatory T cells in patients who received therapeutic cytokine-induced killer (CIK) cells may inhibit host immunity, leading to failed immunotherapy. In this study, we investigated the impact of using interleukin-6 (IL-6) on the phenotype alteration, proliferation, and cytotoxic activity of CIK cells generated from the peripheral blood mononuclear cells of patients with hepatocellular carcinoma. We found that addition of IL-6 to CIK-cell culture medium decreased the percentage of Treg/CD4, Treg/CD3 T cells in the resultant CIK cells and simultaneously increased the proliferation ability, the expression of CD45ROCD62LCCR7 effector memory phenotype, and cytotoxicity of the CIK cells against hepatocellular carcinoma in vitro. Our results also showed that the percentage of Th17/CD4 cells was increased in CIK cells, but the proportion of Th17/CD4 cells was not affected by the addition of IL-6 to CIK-cell culture medium. Collectively, these data suggest that IL-6 may have the potential to improve antitumor activity of CIK cells in cancer immunotherapy.
Authors:
Guohe Lin; Jin Wang; Xiangming Lao; Jun Wang; Lian Li; Shuhong Li; Jinye Zhang; Yong Dong; Alfred E Chang; Qiao Li; Shengping Li
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunotherapy (Hagerstown, Md. : 1997)     Volume:  35     ISSN:  1537-4513     ISO Abbreviation:  J. Immunother.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706083     Medline TA:  J Immunother     Country:  United States    
Other Details:
Languages:  eng     Pagination:  337-43     Citation Subset:  IM    
Affiliation:
*State Key Laboratory of Oncology in Southern China, Department of Hepatobiliary Oncology, Cancer Center ‡State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou †Department of Oncology, Dongguan ShiLong People's Hospital, Dongguan, China §Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI.
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