Document Detail


Interleukin-4 receptor expression in vivo on human AIDS-related Kaposi's sarcoma.
MedLine Citation:
PMID:  9495455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have investigated the expression of interleukin-4 receptors (IL-4R) in acquired immunodeficiency syndrome (AIDS)-related Kaposi's Sarcoma (KS) in situ by immunohistochemistry. Frozen and fixed sections from five patch stage and two nodular stage KS lesions were stained with anti-IL-4R monoclonal antibody with similar results. Skin biopsies from the clinically apparent lesions and adjacent clinically uninvolved skin were also examined. We observed that individual KS cells lining the irregular vascular spaces were stained with anti-IL-4R antibody, although the degree of staining was variable. The epithelioid and oval cells appear to stain more than the spindle cells in plaque stages or nodular lesions. The sections from nonclinically involved skin also contained a few cells with features of KS, singly or in clusters that also stained for IL-4R. Skin sections from four normal donors did not stain with IL-4R antibody except for hair follicles, sweat glands, and faint staining of blood vessels. KS sections were also stained with antibodies to basic fibroblast growth factor (FGF), S100, fibronectin, and von Willebrand factor. KS lesions from clinically involved and uninvolved skin sections were positive for all four antibodies. Thus, the differences between KS lesion and clinically uninvolved skin adjacent to a KS lesion may be more quantitative than qualitative. The IL-4 receptors on KS cells were functional as IL-4 modulated intercellular adhesion molecule 1 (ICAM-1) on these cells. Taken together, our results suggest that AIDS-KS cells express elevated levels of IL-4R compared to normal endothelial and skin cells and, thus, the receptors for IL-4 on KS may serve as an attractive target for anticancer therapy.
Authors:
F Varricchio; S R Husain; P Leland; P Gill; R K Puri
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oncology research     Volume:  9     ISSN:  0965-0407     ISO Abbreviation:  Oncol. Res.     Publication Date:  1997  
Date Detail:
Created Date:  1998-04-09     Completed Date:  1998-04-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9208097     Medline TA:  Oncol Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  495-503     Citation Subset:  IM; X    
Affiliation:
Division of Biostatistics and Epidemiology, Center for Biologics Evaluation and Research, FDA, Rockville, MD, USA.
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MeSH Terms
Descriptor/Qualifier:
Acquired Immunodeficiency Syndrome / complications,  metabolism*,  pathology
Antibodies, Monoclonal
Fibroblast Growth Factor 2 / metabolism
Flow Cytometry
Humans
Immunohistochemistry
Intercellular Adhesion Molecule-1 / metabolism
Interleukin-4 / pharmacology
Neoplasm Proteins / metabolism*
RNA, Messenger / metabolism*
Receptors, Interleukin-4 / immunology,  metabolism*
Sarcoma, Kaposi / complications,  metabolism*,  pathology
Skin / metabolism
Skin Neoplasms / complications,  metabolism*,  pathology
Tumor Cells, Cultured
Up-Regulation
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Neoplasm Proteins; 0/RNA, Messenger; 0/Receptors, Interleukin-4; 103107-01-3/Fibroblast Growth Factor 2; 126547-89-5/Intercellular Adhesion Molecule-1; 207137-56-2/Interleukin-4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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