| Interleukin-27 as a negative regulator of human neutrophil function. | |
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MedLine Citation:
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PMID: 20883313 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interleukin-27 (IL-27) is a novel cytokine of the IL-6/12 family with a broad range of immune regulation properties, which has been considered as a potential therapeutic agent for immune diseases and cancers. However, little is known about the effect of IL-27 on human neutrophils before its clinical administration. In this study, we investigated the effects of IL-27 on human neutrophil functions including adhesion, reactive oxygen species (ROS)/cytotoxic granule components production, inflammatory cytokines production, major histocompatibility complex (MHC) molecules expression and neutrophils' survival. We showed that IL-27 receptor complex, WSX-1/TCCR and gp130, is constitutively expressed on human neutrophils. In vitro, IL-27 suppressed neutrophil adhesion in response to fMLP, which might depend on the down-regulation of Mac-1. IL-27 also suppressed lipopolysaccharide-induced ROS production and attenuated cytotoxic granule components production in the cytoplasm of human neutrophils. In addition, IL-27 enhanced the production of IL-1β but not TNF-α from neutrophils. However, IL-27 failed to regulate the expression of MHC molecules and the survival of human neutrophils. In conclusion, our data demonstrate that IL-27 mainly down-modulates human neutrophil function, which might extend our understanding of the role of IL-27 in the innate immune response. |
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Authors:
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J P Li; H Wu; W Xing; S G Yang; S H Lu; W T Du; J X Yu; F Chen; L Zhang; Z C Han |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of immunology Volume: 72 ISSN: 1365-3083 ISO Abbreviation: Scand. J. Immunol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-04 Completed Date: 2010-11-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0323767 Medline TA: Scand J Immunol Country: England |
Other Details:
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Languages: eng Pagination: 284-92 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Scandinavian Journal of Immunology © 2010 Blackwell Publishing Ltd. |
Affiliation:
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State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Adhesion
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drug effects Cell Nucleus / drug effects, ultrastructure Cell Survival / drug effects Cells, Cultured Cytokine Receptor gp130 / genetics, metabolism Cytokines / metabolism* Cytoplasmic Granules / drug effects, metabolism Flow Cytometry Gene Expression Regulation / drug effects Humans Inflammation Mediators / metabolism Interleukin-1beta / metabolism Interleukins / pharmacology* Lymphocyte Function-Associated Antigen-1 / genetics, metabolism Macrophage-1 Antigen / genetics, metabolism Microscopy, Electron, Transmission Mitochondria / drug effects, ultrastructure Neutrophils / cytology, drug effects*, metabolism Reactive Oxygen Species / metabolism* Receptors, Interleukin / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha / metabolism |
| Chemical | |
Reg. No./Substance:
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0/C19orf10 protein, human; 0/Cytokines; 0/IL27RA protein, human; 0/IL6ST protein, human; 0/Inflammation Mediators; 0/Interleukin-1beta; 0/Interleukins; 0/Lymphocyte Function-Associated Antigen-1; 0/Macrophage-1 Antigen; 0/Reactive Oxygen Species; 0/Receptors, Interleukin; 0/Tumor Necrosis Factor-alpha; 133483-10-0/Cytokine Receptor gp130 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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