Document Detail


Interleukin 2 is not sufficient for the continuous growth of cloned NK-like cytotoxic cell lines.
MedLine Citation:
PMID:  6605387     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interleukin 2 (IL 2) has been strongly implicated as the agent responsible for the continuous growth of T cell lines in vitro. In the present study we confirmed that IL 2 alone could support the growth of a widely used cytotoxic T cell line. In contrast, we found that IL 2 was not sufficient to support the long-term growth of cloned NK-like cytotoxic lymphocyte cell lines. Whereas such lines would grow indefinitely in concanavalin A-induced mouse spleen cell supernatant, they would only grow for short periods (2 to 3 days) in the IL 2-containing supernatant of phytohemagglutinin-stimulated LBRM-33 tumor cells, or in IL 2 partially purified from spleen cell or LBRM-33 supernatants. The addition of concanavalin-A or interferon (type beta or gamma) to these supernatants did not improve growth. By contrast, the NK-like cells proliferated equally well in a short-term (24-hr) assay, irrespective of the source of IL 2 (spleen or LBRM-33 supernatant, or partially purified IL 2). Furthermore, the NK-like cells readily depleted IL 2 from the medium, either during growth at 37 degrees C or by absorption at 4 degrees C. It is concluded that at least some cytotoxic cell lines require both IL 2 and other, as yet unidentified, spleen cell-derived factors for long-term growth.
Authors:
S E Olabuenaga; C G Brooks; S Gillis; C S Henney
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  131     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1983-12-17     Completed Date:  1983-12-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2386-91     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
Cell Line
Cell Survival
Clone Cells / cytology
Concanavalin A / pharmacology,  physiology
Culture Media
Interleukin-2 / physiology*
Killer Cells, Natural / cytology*
Lymphokines*
Mice
Mice, Inbred BALB C
Mice, Inbred DBA
Rats
Rats, Inbred WF
T-Lymphocytes, Cytotoxic / cytology*
Time Factors
Grant Support
ID/Acronym/Agency:
AI-15384/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Interleukin-2; 0/Lymphokines; 0/concanavalin A-induced helper factors; 11028-71-0/Concanavalin A

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