Document Detail

Interleukin-2 and a T cell hybridoma (MP6) derived B cell-stimulatory factor act synergistically to induce proliferation and differentiation of human B-chronic lymphocytic leukemia cells.
MedLine Citation:
PMID:  2787454     Owner:  NLM     Status:  MEDLINE    
In this paper we communicate that cells of a selected B-CLL clone (I83), after 2 days of Staphylococcus aureus Cowan strain 1 (SAC) activation, respond to recombinant IL-2 (rIL-2) and a B cell stimulatory factor (BSF-MP6) and act in strong synergism with induction of simultaneous high-rate proliferation and differentiation. None of the factors alone or other lymphokines (IFN-gamma, TNF-alpha, 12 kDa BCGF, IL-1, IL-4, IL-5, IL-6) induced significant DNA synthesis in SAC-activated cells. However, low levels of IgM were produced by cells stimulated by SAC + rIL-2. The SAC activation was followed by an increase in IL-2 receptor (IL-2R; CD25) expression, and the proliferation induced by BSF-MP6 + rIL-2 could be blocked in a dose-dependent manner by alpha-CD25 antibody. Furthermore, flow cytometric cell cycle studies showed that SAC and BSF-MP6 + rIL-2 stimulated cells underwent a complete transition through the cell cycle to become arrested in G1. The induced proliferation by BSF-MP6 + rIL-2 was dependent on serum but independent of the 2.8% of CD4, CD8, CD14, and CD16 positive cells contaminating the I83 cell population. Previously, we reported that I83 cells activated by 12-O-tetradecanoylphorbol-13-acetate (TPA) were induced to differentiation only but that the addition of BSF-MP6 induced DNA synthesis concomitantly with the differentiation. This paper demonstrates that physiological stimuli can induce both high-rate proliferation and differentiation in a B-CLL clone in vitro. It also suggests that the low proliferation and the differentiation block in vivo, characteristic of most B-CLLs, may reflect a subnormal response of B-CLL cells to growth and differentiation factors, or a dysfunction in the factor production by the patients' T cells.
M Carlsson; T H Tötterman; A Rosén; K Nilsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia     Volume:  3     ISSN:  0887-6924     ISO Abbreviation:  Leukemia     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-08-18     Completed Date:  1989-08-18     Revised Date:  2013-03-04    
Medline Journal Info:
Nlm Unique ID:  8704895     Medline TA:  Leukemia     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  593-601     Citation Subset:  IM    
Department of Pathology, University Hospital, Uppsala, Sweden.
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MeSH Terms
Cell Cycle / drug effects
Cell Differentiation / drug effects
Cell Division / drug effects
DNA / biosynthesis*
Dose-Response Relationship, Drug
Drug Synergism
Interleukin-2 / pharmacology*
Interleukins / pharmacology*
Leukemia, Lymphocytic, Chronic, B-Cell / pathology*
Receptors, Interleukin-2 / analysis
Recombinant Proteins / pharmacology
Reg. No./Substance:
0/Interleukin-2; 0/Interleukins; 0/Receptors, Interleukin-2; 0/Recombinant Proteins; 207137-56-2/Interleukin-4; 9007-49-2/DNA
Comment In:
Leukemia. 1990 Mar;4(3):238-9   [PMID:  2179641 ]

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