| Interleukin-2 and a T cell hybridoma (MP6) derived B cell-stimulatory factor act synergistically to induce proliferation and differentiation of human B-chronic lymphocytic leukemia cells. | |
| | |
MedLine Citation:
|
PMID: 2787454 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In this paper we communicate that cells of a selected B-CLL clone (I83), after 2 days of Staphylococcus aureus Cowan strain 1 (SAC) activation, respond to recombinant IL-2 (rIL-2) and a B cell stimulatory factor (BSF-MP6) and act in strong synergism with induction of simultaneous high-rate proliferation and differentiation. None of the factors alone or other lymphokines (IFN-gamma, TNF-alpha, 12 kDa BCGF, IL-1, IL-4, IL-5, IL-6) induced significant DNA synthesis in SAC-activated cells. However, low levels of IgM were produced by cells stimulated by SAC + rIL-2. The SAC activation was followed by an increase in IL-2 receptor (IL-2R; CD25) expression, and the proliferation induced by BSF-MP6 + rIL-2 could be blocked in a dose-dependent manner by alpha-CD25 antibody. Furthermore, flow cytometric cell cycle studies showed that SAC and BSF-MP6 + rIL-2 stimulated cells underwent a complete transition through the cell cycle to become arrested in G1. The induced proliferation by BSF-MP6 + rIL-2 was dependent on serum but independent of the 2.8% of CD4, CD8, CD14, and CD16 positive cells contaminating the I83 cell population. Previously, we reported that I83 cells activated by 12-O-tetradecanoylphorbol-13-acetate (TPA) were induced to differentiation only but that the addition of BSF-MP6 induced DNA synthesis concomitantly with the differentiation. This paper demonstrates that physiological stimuli can induce both high-rate proliferation and differentiation in a B-CLL clone in vitro. It also suggests that the low proliferation and the differentiation block in vivo, characteristic of most B-CLLs, may reflect a subnormal response of B-CLL cells to growth and differentiation factors, or a dysfunction in the factor production by the patients' T cells. |
| | |
Authors:
|
M Carlsson; T H Tötterman; A Rosén; K Nilsson |
Related Documents
:
|
8898244 - Kinase-negative mutant epidermal growth factor receptor (egfr) expression during embryo... 15596414 - C2c12 murine myoblasts as a model of skeletal muscle development: morpho-functional cha... 8297384 - Erythroid differentiation-inducing activity of protoporphyrin ix and its analogs on hum... 3028094 - Myeloid differentiated hl-60 cells display specific leukotriene b4 binding sites. 23540554 - Comparison of x-ray and alpha particle effects on a human cancer and endothelial cells:... 21881604 - Cell death in disease: from 2010 onwards. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Leukemia Volume: 3 ISSN: 0887-6924 ISO Abbreviation: Leukemia Publication Date: 1989 Aug |
Date Detail:
|
Created Date: 1989-08-18 Completed Date: 1989-08-18 Revised Date: 2013-03-04 |
Medline Journal Info:
|
Nlm Unique ID: 8704895 Medline TA: Leukemia Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 593-601 Citation Subset: IM |
Affiliation:
|
Department of Pathology, University Hospital, Uppsala, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Cycle
/
drug effects Cell Differentiation / drug effects Cell Division / drug effects DNA / biosynthesis* Dose-Response Relationship, Drug Drug Synergism Humans Interleukin-2 / pharmacology* Interleukin-4 Interleukins / pharmacology* Leukemia, Lymphocytic, Chronic, B-Cell / pathology* Receptors, Interleukin-2 / analysis Recombinant Proteins / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Interleukin-2; 0/Interleukins; 0/Receptors, Interleukin-2; 0/Recombinant Proteins; 207137-56-2/Interleukin-4; 9007-49-2/DNA |
| Comments/Corrections | |
Comment In:
|
Leukemia. 1990 Mar;4(3):238-9
[PMID:
2179641
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Functional and molecular characterization of B cell line derived interleukin-1 alpha.
Next Document: Induction of tumoricidal activity and alterations of growth by interleukin-2 and manipulation of pro...