Document Detail

Interleukin-1beta isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines.
MedLine Citation:
PMID:  11683587     Owner:  NLM     Status:  MEDLINE    
The gilthead seabream IL-1beta gene consists of five exons/four introns. The complete coding sequence contains a 102 bp 5' untranslated region (UTR), a single open reading frame of 762 bp which translates into a 253 amino acid molecule, and a 407 bp 3'UTR with a polyadenylation signal 14 nucleotides upstream of the poly(A)tail. The seabream sequence has the highest degree of nucleotide (61.7%) and amino acid (53%) identity with the trout IL-1beta sequences. The IL-1beta message was detected by RT-PCR in head-kidney, blood, spleen, liver, gill and peritoneal exudate of both non-infected and Vibrio anguillarum-challenged fish. More importantly, IL-1beta was highly expressed by purified macrophage monolayers and was up-regulated by lipopolysaccharide and lymphocyte-derived macrophage-activating factor stimulation.
P Pelegrín; J García-Castillo; V Mulero; J Meseguer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytokine     Volume:  16     ISSN:  1043-4666     ISO Abbreviation:  Cytokine     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-30     Completed Date:  2002-02-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  67-72     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Interleukin-1 / chemistry,  genetics,  metabolism*
Lipopolysaccharides / pharmacology*
Lymphokines / pharmacology*
Macrophage Activation*
Macrophages / drug effects,  immunology*
Molecular Sequence Data
Organ Specificity
Reverse Transcriptase Polymerase Chain Reaction
Sea Bream / genetics,  immunology*,  microbiology
Sequence Alignment
Reg. No./Substance:
0/Interleukin-1; 0/Lipopolysaccharides; 0/Lymphokines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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