Document Detail

Interleukin-1beta-induced nitric oxide production in rat aortic endothelial cells: inhibition by estradiol in normal and high glucose cultures.
MedLine Citation:
PMID:  10403504     Owner:  NLM     Status:  MEDLINE    
Expression of inducible nitric oxide synthase (iNOS) and the resultant increased nitric oxide (NO) production are associated with septic shock, atherosclerosis, and cytokine-induced vascular injury. Estrogen is known to impact vascular injury and vascular tone, in part through regulation of NO production. In the current study, we examined the effect of physiological concentrations of estradiol on interleukin-1beta (IL-1beta)-induced NO production in rat aortic endothelial cells (RAECs). 17Beta-estradiol significantly decreased IL-1beta-induced iNOS protein levels and reduced NO production in RAECs. High glucose (25 mM) elevated the increase in IL-1beta-induced iNOS protein and NO production. Nevertheless, estradiol still inhibited IL-1beta-induced iNOS and NO production even in the presence of high glucose. These data suggest that estradiol may exert its beneficial effects in part by inhibiting induction of endothelial iNOS, a possible mechanism for the protective effect of estradiol against diabetes-associated cardiovascular complications.
R Xu; J A Morales; R Muniyappa; D F Skafar; J L Ram; J R Sowers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Life sciences     Volume:  64     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1999  
Date Detail:
Created Date:  1999-07-22     Completed Date:  1999-07-22     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2451-62     Citation Subset:  IM    
Department of Physiology, Wayne State University, Detroit, MI 48201, USA.
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MeSH Terms
Blotting, Western
Cells, Cultured
Dose-Response Relationship, Drug
Endothelium, Vascular / cytology,  drug effects*,  enzymology,  metabolism
Enzyme Induction / drug effects
Estradiol / analogs & derivatives,  pharmacology*
Estradiol Antagonists / pharmacology
Glucose / pharmacology*
Interleukin-1 / antagonists & inhibitors*,  pharmacology
Nitric Oxide / metabolism*
Nitric Oxide Synthase / metabolism
Nitric Oxide Synthase Type II
Reg. No./Substance:
0/Estradiol Antagonists; 0/Interleukin-1; 10102-43-9/Nitric Oxide; 22X328QOC4/fulvestrant; 50-28-2/Estradiol; 50-99-7/Glucose; EC Oxide Synthase; EC Oxide Synthase Type II; EC protein, rat

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