| Interleukin-1beta-induced growth enhancement of Staphylococcus aureus occurs in biofilm but not planktonic cultures. | |
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MedLine Citation:
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PMID: 16769197 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Staphylococcus aureus causes recalcitrant infections and forms resistant biofilms. Mechanisms of biofilm resistance to host defenses may include changes in gene expression that confer responsiveness to chemical mediators. In earlier studies fresh clinical isolates responded to inflammatory cytokines, but responsiveness was lost after multiple in vitro passages [Meduri et al. Cytokines IL-1beta, IL-6, and TNF-alpha enhance the In vitro growth of bacteria. Am J Respir Crit Care Med 1999;160:961-7]. Since biofilms more closely resemble in vivo growth and are implicated in recalcitrant infections, we hypothesized that biofilms, but not planktonic cells, would respond to cytokines. Biofilms were induced by ethanol in S. aureus ATCC 12600. Biofilms treated with 2 ng/mL interleukin-1beta (IL-1beta) for 6 h contained 2.5-fold more cells than untreated biofilms, but no growth-enhancement occurred in planktonic cultures. As determined by flow cytometry, IL-beta bound to 63.1% of biofilm cells, but only 11.2% of planktonic cells. Our results provide evidence of a differential response of biofilm and planktonic bacteria to chemical mediators, and suggest that biofilm bacteria may evade host defenses by growing more rapidly in response to the inflammatory mediators released by activated host defense cells. |
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Authors:
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Renee A McLaughlin; Arlene J Hoogewerf |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Microbial pathogenesis Volume: 41 ISSN: 0882-4010 ISO Abbreviation: Microb. Pathog. Publication Date: 2006 Aug-Sep |
Date Detail:
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Created Date: 2006-09-27 Completed Date: 2006-11-02 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8606191 Medline TA: Microb Pathog Country: England |
Other Details:
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Languages: eng Pagination: 67-79 Citation Subset: IM |
Affiliation:
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Department of Biology, Calvin College, 1726 Knollcrest Circle SE, Grand Rapids, MI 49546-4403, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriological Techniques Biofilms / drug effects*, growth & development* Chemokine CCL4 Dose-Response Relationship, Drug Flow Cytometry / methods Interleukin-1 / metabolism, pharmacology* Interleukin-6 / pharmacology Macrophage Inflammatory Proteins / pharmacology Polysaccharides, Bacterial / biosynthesis, genetics Staphylococcus aureus / drug effects*, metabolism, physiology* Tumor Necrosis Factor-alpha / pharmacology Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL4; 0/Interleukin-1; 0/Interleukin-6; 0/Macrophage Inflammatory Proteins; 0/Polysaccharides, Bacterial; 0/Tumor Necrosis Factor-alpha; 0/polysaccharide intercellular adhesin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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