Document Detail


Interleukin-1beta (IL-1beta) is a modulator of human luteal cell steroidogenesis: localization of the IL type I system in the corpus luteum.
MedLine Citation:
PMID:  10566679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present investigation examined the effect of interleukin-1beta (IL-1beta) on progesterone production by human luteal cells and the expression and localization of the IL-1 system in the human corpus luteum (CL). Luteal cells were isolated from corpora lutea collected throughout the luteal phase. After dispersion, luteal cells were treated with a panel of monoclonal antibodies directed to leukocyte-specific molecules. The leukocytes were isolated with immunomagnetic beads. Leukocyte-free luteal cells exhibited greater steroidogenic responsiveness to hCG toward the end of the luteal phase. The treatment of mixed luteal cells (total luteal cells) with IL-1beta inhibited by 60% hCG-stimulated progesterone production. Interestingly, the treatment of leukocyte-free luteal cells with IL-1beta did not affect progesterone production. In addition, the treatment of mixed luteal cells with monoclonal antibodies against IL-1 receptor type I (IL-1RtI) resulted in a 2.5-fold increase in the hCG-supported progesterone production. IL-1RtI and IL-1 receptor antagonist were localized by immunohistochemistry in both somatic and immune cells of the CL. Flow cytometric analysis indicated that both nonleukocyte luteal cells and leukocyte-luteal cells exhibited IL-1Rt-I positive cells, representing 56% and 31% of the total luteal cells, respectively. However, 13% of nonleukocyte luteal cells did not express IL-1Rt-I. Northern analysis demonstrated the presence of the 5.1-kb IL-1RtI messenger ribonucleic acid transcript in CL of different ages. RT-PCR indicated that both leukocyte-free luteal cells and luteal leukocytes express IL-1RtI messenger ribonucleic acid. We conclude that 1) luteal leukocytes have an inhibitory effect on hCG-stimulated progesterone production; 2) IL-1beta inhibits hCG-stimulated progesterone production only in mixed luteal cell cultures, indicating that leukocytes mediate the effect; 3) the somatic and immune cells of the CL are sites of action and expression of the IL-1 system; and 4) interaction between the steroidogenic and immune cells of the CL suggests a functional intraovarian role for IL-1beta in CL physiology.
Authors:
P Kohen; A Castro; P Caballero-Campo; O Castro; M Vega; A Makrigiannakis; C Simón; P Carvallo; L Devoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  84     ISSN:  0021-972X     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-11-26     Completed Date:  1999-11-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4239-45     Citation Subset:  AIM; IM    
Affiliation:
Institute of Maternal and Child Research, School of Medicine and Department of Obstetrics and Gynecology, University of Chile, Santiago.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Monoclonal / pharmacology
Chorionic Gonadotropin / pharmacology
Corpus Luteum / chemistry,  cytology,  metabolism*
Female
Flow Cytometry
Humans
Immunohistochemistry
Interleukin-1 / pharmacology*
Kinetics
Leukocytes / chemistry,  physiology
Luteal Cells / metabolism*
Luteal Phase
Progesterone / biosynthesis*
RNA, Messenger / analysis
Receptors, Interleukin-1 / analysis,  genetics,  physiology
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Chorionic Gonadotropin; 0/Interleukin-1; 0/RNA, Messenger; 0/Receptors, Interleukin-1; 57-83-0/Progesterone

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