| Interleukin-1alpha is a regulator of the blood-testis barrier. | |
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MedLine Citation:
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PMID: 21191089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Throughout spermatogenesis, the Sertoli cell blood-testis barrier (BTB) is strictly regulated by cytokines, which mediate its timely restructuring, thereby allowing spermatocytes to enter the adluminal compartment of the seminiferous epithelium for development into spermatozoa. The aim herein was to investigate whether germ cells play a role in BTB restructuring via the action of interleukin-1α (IL-1α) since germ cells are known to control Sertoli cell production of this cytokine, and if yes, how these effects are mediated. When Sertoli cells were isolated from Sprague-Dawley rats and plated at high density, IL-1α (100 pg/ml) was shown to "open" the Sertoli cell barrier when its integrity was assessed by transepithelial electrical resistance measurements. Further investigation of Sertoli cells treated with IL-1α revealed striking changes in the cellular distribution of actin filaments when compared to untreated cells. These effects at the Sertoli cell barrier were mediated, in part, by epidermal growth factor receptor pathway substrate 8 (Eps8; an actin bundling and barbed-end capping protein) and actin-related protein 3 (Arp3; a component of the actin nucleation machinery). As important, an increase in the kinetics of occludin internalization but a decrease in its rate of degradation was noted following IL-1α treatment. These results indicate that IL-1α is a critical regulator of BTB dynamics. |
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Authors:
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Pearl P Y Lie; C Yan Cheng; Dolores D Mruk |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-12-29 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 25 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-01 Completed Date: 2011-06-03 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 1244-53 Citation Subset: IM |
Affiliation:
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Center for Biomedical Research, Population Council, New York, NY 10065, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actin Cytoskeleton
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drug effects Animals Blood-Testis Barrier / drug effects* Cells, Cultured Endocytosis / drug effects Interleukin-1alpha / pharmacology, physiology* Male Membrane Proteins / metabolism Occludin Phosphoproteins / metabolism Rats Rats, Sprague-Dawley Sertoli Cells / drug effects Spermatogenesis / drug effects Tight Junctions / drug effects Zonula Occludens-1 Protein |
| Grant Support | |
ID/Acronym/Agency:
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R01 HD056034/HD/NICHD NIH HHS; R01 HD056034-02S1/HD/NICHD NIH HHS; R03 HD061401/HD/NICHD NIH HHS; U54 HD029990/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1alpha; 0/Membrane Proteins; 0/Occludin; 0/Ocln protein, rat; 0/Phosphoproteins; 0/Tjp1 protein, rat; 0/Zonula Occludens-1 Protein |
| Comments/Corrections | |
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