Document Detail

Interleukin 1alpha and interleukin 6 protect human neuronal SH-SY5Y cells from oxidative damage.
MedLine Citation:
PMID:  15135888     Owner:  NLM     Status:  MEDLINE    
Interleukin (IL)-1alpha and IL-6 are powerful inflammatory cytokines produced in brain primarily by microglia and astrocytes. Here we demonstrate, using an in vitro assay system, that they can have a direct neuroprotective action against oxidative attack. Exposure of retinoic acid-differentiated human SH-SY5Y neuroblastoma cells to 270 microM hydrogen peroxide caused activation of caspase 3 and significant neuronal death. Treatment with IL-1alpha or IL-6 caused a dose-dependent increase in survival as measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. An antibody against single-strand DNA demonstrated many apoptotic neuroblastoma cells following exposure to hydrogen peroxide, with a decrease following cytokine treatment. These data indicate that IL-1alpha and IL-6 can, under appropriate circumstances, protect neurons from oxidative damage in addition to their well-known action of stimulating inflammation.
Christopher J Bissonnette; Andis Klegeris; Patrick L McGeer; Edith G McGeer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  361     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-11     Completed Date:  2004-08-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  40-3     Citation Subset:  IM    
Kinsmen Laboratory of Neurological Research, University of British Columbia, Vancouver, BC, Canada.
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MeSH Terms
Apoptosis / drug effects,  immunology
Caspase 3
Caspases / drug effects,  metabolism
Cell Line, Tumor
Cell Survival / drug effects,  immunology
DNA, Single-Stranded / drug effects
Dose-Response Relationship, Drug
Encephalitis / immunology,  physiopathology
Hydrogen Peroxide
Interleukin-1 / pharmacology*,  therapeutic use
Interleukin-6 / pharmacology*,  therapeutic use
Nerve Degeneration / drug therapy*,  immunology,  physiopathology
Neurons / drug effects*,  metabolism
Neuroprotective Agents / pharmacology*,  therapeutic use
Oxidative Stress / drug effects*,  physiology
Reg. No./Substance:
0/DNA, Single-Stranded; 0/Interleukin-1; 0/Interleukin-6; 0/Neuroprotective Agents; 7722-84-1/Hydrogen Peroxide; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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