| Interleukin-17A Contributes to Myocardial Ischemia/Reperfusion Injury by Regulating Cardiomyocyte Apoptosis and Neutrophil Infiltration. | |
| | |
MedLine Citation:
|
PMID: 22261166 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
OBJECTIVES: This study tested whether interleukin (IL)-17A is involved in the pathogenesis of mouse myocardial ischemia/reperfusion (I/R) injury and investigated the mechanisms. BACKGROUND: Inflammatory processes play a major role in myocardial I/R injury. We recently identified IL-17A as an important cytokine in inflammatory cardiovascular diseases such as atherosclerosis and viral myocarditis. However, its role in myocardial I/R injury remains unknown. METHODS: The involvement of IL-17A was assessed in functional assays in mouse myocardial I/R injury by neutralization/repletion or genetic deficiency of IL-17A, and its mechanism on cardiomyocyte apoptosis and neutrophil infiltration were further studied in vivo and in vitro. RESULTS: Interleukin-17A was elevated after murine left coronary artery ligation and reperfusion. Intracellular cytokine staining revealed that γδT lymphocytes but not CD4(+) helper T cells were a major source of IL-17A. Anti-IL-17A monoclonal antibody treatment or IL-17A knockout markedly ameliorated I/R injury, as demonstrated by reduced infarct size, reduced cardiac troponin T levels, and improved cardiac function. This improvement was associated with a reduction in cardiomyocyte apoptosis and neutrophil infiltration. In contrast, repletion of exogenous IL-17A induced the opposite effect. In vitro study showed that IL-17A mediated cardiomyocyte apoptosis through regulating the Bax/Bcl-2 ratio, induced CXC chemokine-mediated neutrophil migration and promoted neutrophil-endothelial cell adherence through induction of endothelial cell E-selectin and inter-cellular adhesion molecule-1 expression. CONCLUSIONS: IL-17A mainly produced by γδT cells plays a pathogenic role in myocardial I/R injury by inducing cardiomyocyte apoptosis and neutrophil infiltration. |
| | |
Authors:
|
Yu-Hua Liao; Ni Xia; Su-Feng Zhou; Ting-Ting Tang; Xin-Xin Yan; Bing-Jie Lv; Shao-Fang Nie; Jing Wang; Yoichiro Iwakura; Hong Xiao; Jing Yuan; Harish Jevallee; Fen Wei; Guo-Ping Shi; Xiang Cheng |
Related Documents
:
|
9171306 - Regulation of mhc class i expression by inflammatory cytokines in rat mesangial cells. 1386516 - A molecular model of mhc class-i-restricted antigen processing. 21749416 - Cattle and chemokines: evidence for species-specific evolution of the bovine chemokine ... 20360936 - Pheophorbide a-mediated photodynamic therapy triggers hla class i-restricted antigen pr... 16563426 - Use of bone marrow-derived macrophages to model murine innate immune responses. 22354026 - Induction of protective immunity against eimeria tenella, eimeria maxima, and eimeria a... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of the American College of Cardiology Volume: 59 ISSN: 1558-3597 ISO Abbreviation: J. Am. Coll. Cardiol. Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-01-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8301365 Medline TA: J Am Coll Cardiol Country: United States |
Other Details:
|
Languages: eng Pagination: 420-9 Citation Subset: AIM; IM |
Copyright Information:
|
Copyright © 2012 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved. |
Affiliation:
|
Institute of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transcriptome characterization of estrogen-treated human myocardium identifies Myosin regulatory lig...
Next Document: Deviation from Murray's law is associated with a higher degree of calcification in coronary bifurcat...