Document Detail


Interleukin-15 inhibits smooth muscle cell proliferation and hyaluronan production in rat ductus arteriosus.
MedLine Citation:
PMID:  17667861     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neointimal cushion formation (NCF) is an important vascular remodeling for anatomical closure of the ductus arteriosus (DA). Inflammatory responses to vascular injury or atherosclerosis are known to be associated with the pathogenesis of NCF. We found that the expression of interleukin (IL)-15 mRNA was significantly higher in rat DA than in the aorta. IL-15 immunoreactivity was detected predominantly in the internal elastic laminae (IEL) and to a lesser extent in smooth muscle cells (SMCs) in rat DA. Prostaglandin E (PGE) increased the expression of IL-15 mRNA in cultured DA SMCs. IL-15 significantly attenuated the platelet-derived growth factor (PDGF)-BB-mediated SMC proliferation, but did not change SMC migration. IL-15 significantly attenuated PGE1-induced hyaluronic acid (HA) production in a dose-dependent manner, which is a potent stimulator of NCF. Accordingly, IL-15 might have an inhibitory effect on the physiologic vascular remodeling processes in closing the DA.
Authors:
Shiho Iwasaki; Susumu Minamisawa; Utako Yokoyama; Toru Akaike; Hong Quan; Yoji Nagashima; Shigeru Nishimaki; Yoshihiro Ishikawa; Shumpei Yokota
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric research     Volume:  62     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-24     Completed Date:  2007-11-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  392-8     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Yokohama City University, Yokohama 236-0004, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alprostadil / metabolism
Animals
Aorta / embryology,  metabolism*
Cell Movement
Cell Proliferation*
Cells, Cultured
Chemokine CX3CL1
Chemokines, CX3C / metabolism
Dose-Response Relationship, Drug
Ductus Arteriosus / embryology,  metabolism*
Feedback, Physiological
Gene Expression Regulation, Developmental
Hyaluronic Acid / metabolism*
Interleukin-15 / genetics,  metabolism*,  pharmacology
Membrane Proteins / metabolism
Methyl Ethers / pharmacology
Muscle, Smooth, Vascular / embryology,  metabolism*
Myocytes, Smooth Muscle / metabolism*
Platelet-Derived Growth Factor / metabolism
RNA, Messenger / metabolism
Rats
Rats, Wistar
Receptors, Chemokine / metabolism
Receptors, Interleukin-15 / metabolism
Receptors, Prostaglandin E / metabolism
Chemical
Reg. No./Substance:
0/CX3CR1 protein, rat; 0/Chemokine CX3CL1; 0/Chemokines, CX3C; 0/Cx3cl1 protein, rat; 0/Interleukin-15; 0/Membrane Proteins; 0/Methyl Ethers; 0/ONO-AE1-329; 0/Platelet-Derived Growth Factor; 0/RNA, Messenger; 0/Receptors, Chemokine; 0/Receptors, Interleukin-15; 0/Receptors, Prostaglandin E; 0/platelet-derived growth factor BB; 0/prostaglandin E2 receptor, EP4 subtype; 745-65-3/Alprostadil; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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