Document Detail


Interleukin 13 and inflammatory markers in human sepsis.
MedLine Citation:
PMID:  15164448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Interleukin (IL) 13 is an anti-inflammatory cytokine that reduces inflammatory cytokine production, and enhances monocyte survival and MHC class II and CD23 expression. The only report of IL-13 in human sepsis noted no increase in IL-13 concentration, in contrast to animal data. This study further examined the expression of IL-13 in relation to human sepsis. METHODS: In a prospective observational study of 31 patients (24 men) with sepsis or septic shock, high-sensitivity enzyme-linked immunoabsorbent assay (ELISA) was used to quantify levels of tumour necrosis factor (TNF) alpha on admission, and on days 1, 3, 5 and 7 thereafter. IL-13 and IL-2 were assayed by standard ELISA, and HLA-DR on CD14-positive monocytes was measured by flow cytometry. RESULTS: Twenty-three patients developed septic shock. Monocyte HLA-DR levels showed greater depression and a slower recovery in shocked than non-shocked patients. The serum IL-13 concentration was significantly higher in the shocked group from admission to day 3, but subsequently decreased to levels similar to those in the non-shocked group. IL-13 concentrations were higher in non-survivors. The TNF-alpha concentration was higher in those with septic shock than in those without. The TNF-alpha level correlated with IL-13 concentration (r(S) = 0.61, P = 0.002). The IL-13/TNF-alpha ratio was greater in patients with shock than those with sepsis only (P = 0.017). IL-2 was undetectable. CONCLUSION: In human sepsis and septic shock, IL-13 correlated with TNF-alpha expression, but its effect on HLA-DR class II molecules remains unclear.
Authors:
N Collighan; P V Giannoudis; O Kourgeraki; S L Perry; P J Guillou; M C Bellamy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The British journal of surgery     Volume:  91     ISSN:  0007-1323     ISO Abbreviation:  Br J Surg     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-05-27     Completed Date:  2004-06-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372553     Medline TA:  Br J Surg     Country:  England    
Other Details:
Languages:  eng     Pagination:  762-8     Citation Subset:  AIM; IM    
Copyright Information:
Copyright 2004 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd.
Affiliation:
Department of Anaesthesia, St James's University Hospital, Leeds LS9 7TF, UK.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Biological Markers / blood
Enzyme-Linked Immunosorbent Assay / methods
Female
HLA-DR Antigens / metabolism
Humans
Interleukin-13 / blood*
Male
Middle Aged
Prospective Studies
Sepsis / blood*
Shock, Septic / blood*
Tumor Necrosis Factor-alpha / metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/HLA-DR Antigens; 0/Interleukin-13; 0/Tumor Necrosis Factor-alpha

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