| Interleukin-13, but not indomethacin, increases cysteinyl-leukotriene synthesis in human lung macrophages. | |
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MedLine Citation:
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PMID: 22121385 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Aspirin-exacerbated respiratory disease (AERD) is associated with constitutively elevated synthesis of bronchoconstrictor cysteinyl-leukotrienes, associated with increased expression of leukotriene (LT)C(4) synthase and Th2 cytokines and airway eosinophilia. We examined whether interleukin-13 can increase LTC(4) synthase gene transcription and cysteinyl-leukotriene synthesis in macrophages isolated from resected human lung tissue and whether an NSAID (indomethacin) can trigger further cysteinyl-leukotriene synthesis in these cells. Overnight culture of human lung macrophages with IL-13 (10 ng/mL) increased spontaneous and ionophore-stimulated production of cysteinyl-leukotrienes by 42% (P = 0.02) and 52% (P = 0.005), respectively, as quantified by enzyme immunoassays, but PCR gene transcription assays did not demonstrate an effect on LTC4S mRNA. The addition of indomethacin (100 μM) did not modulate cysteinyl-leukotriene production in either IL-13-treated or untreated macrophages. We conclude that while IL-13 enhances cysteinyl-leukotriene synthesis in human lung macrophages, it does not replicate the enhanced LTC(4) synthase expression observed in the AERD lung nor confer sensitivity to NSAIDs. |
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Authors:
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Sarah E Jackson; John W Holloway; Jane A Warner; Anthony P Sampson |
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Publication Detail:
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Type: Journal Article Date: 2011-10-29 |
Journal Detail:
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Title: Journal of allergy Volume: 2012 ISSN: 1687-9791 ISO Abbreviation: J Allergy (Cairo) Publication Date: 2012 |
Date Detail:
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Created Date: 2011-11-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101535174 Medline TA: J Allergy (Cairo) Country: United States |
Other Details:
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Languages: eng Pagination: 348741 Citation Subset: - |
Affiliation:
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Infection, Inflammation and Immunity Division, University of Southampton School of Medicine, Southampton SO16 6YD, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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