Document Detail

Interleukin-10 inhibits antigen-induced cellular recruitment into the airways of sensitized mice.
MedLine Citation:
PMID:  7769104     Owner:  NLM     Status:  MEDLINE    
This report examines the effect of recombinant murine (rm) IL-10 on antigen-induced cellular recruitment into the airways of sensitized Balb/c mice. The intranasal instillation of 10 micrograms ovalbumin induced an early (6-24 h) increase in the number of neutrophils, and a late rise (24-96 h) in that of eosinophils in the bronchoalveolar lavage (BAL) fluid and bronchial tissue. A single intranasal instillation of 0.01-0.1 microgram of rmIL-10, administered concurrently with ovalbumin, but not 1 or 3 h thereafter, dose-dependently inhibited both airway neutrophilia and eosinophilia. This phenomenon was suppressed by treating the sensitized mice with 1 mg/mouse of a neutralizing anti-IL-10 mAb, which increased significantly ovalbumin-induced neutrophil and eosinophil accumulation in the BAL fluid. These results suggest that antigen stimulation may trigger the in vivo generation of IL-10, which, in turn, participates in the leukocyte infiltration into the airways. rmIL-10 also reduced TNF-alpha release in the BAL fluid observed 1 and 3 h after antigen challenge. Furthermore, the intranasal instillation of an anti-TNF-alpha antiserum to sensitized mice markedly reduced ovalbumin-induced neutrophil and eosinophil accumulation in the BAL fluid. These findings indicate that leukocyte infiltration into the airways of antigen-challenged mice is regulated by IL-10. Furthermore, inhibition of TNF-alpha production by rmIL-10 suggests that allergic airway inflammation and TNF-alpha formation are parallel events in this model.
C Zuany-Amorim; S Hailé; D Leduc; C Dumarey; M Huerre; B B Vargaftig; M Pretolani
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  95     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-07-06     Completed Date:  1995-07-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2644-51     Citation Subset:  AIM; IM    
Unité de Pharmacologie Cellulaire, Unité Associée Institut Pasteur/Institut National de la Santé et de la Recherche Médicale No. 285, Paris, France.
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MeSH Terms
Administration, Intranasal
Antigens / immunology
Bronchi / pathology
Bronchoalveolar Lavage Fluid / chemistry,  cytology
Hypersensitivity / pathology,  physiopathology*
Interleukin-10 / pharmacology*
Lung / physiopathology*
Mice, Inbred BALB C
Ovalbumin / immunology
Recombinant Proteins
Tumor Necrosis Factor-alpha / metabolism*
Reg. No./Substance:
0/Antigens; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10; 9006-59-1/Ovalbumin

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