Document Detail

Interleukin-1 and tumor necrosis factor stimulate arachidonic acid release and phospholipid metabolism in human myometrial cells.
MedLine Citation:
PMID:  8238136     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Our aim was to evaluate the effects of the cytokines interleukin-1 and tumor necrosis factor on arachidonic acid release in human myometrial cells. STUDY DESIGN: Primary monolayer cultures of human myometrial cells prelabeled with tritiated arachidonic acid were exposed to interleukin-1 or tumor necrosis factor for varying periods and the release of tritiated arachidonic acid and its loss from phospholipids were measured by radiochromatography. To gain some information on the biologic action of interleukin-1 the contractile response to oxytocin was measured in myometrial strips preincubated with this cytokine. Data were statistically evaluated with analysis of variance or Student's test. RESULTS: Both cytokines caused a dose-dependent increase in tritiated arachidonate release that was suppressed by the protein synthesis inhibitor cycloheximide. Tritiated arachidonic acid release was maximal after 24 hours of stimulation with interleukin-1. Both interleukin-1 and tumor necrosis factor stimulated the release of the isotopically labeled fatty acid from phosphatidylcholine. In addition, interleukin-1 also increased the loss of arachidonic acid from phosphatidic acid and significantly potentiated the oxytocin-evoked myometrial contractility. CONCLUSIONS: Both interleukin-1 and tumor necrosis factor enhance arachidonic acid release, probably by inducing the synthesis of phospholipase A2 and possibly other enzymes involved in the metabolism of phospholipids. In turn, arachidonic acid itself may act as a second messenger, synergizing with other uterotonic agents, as well as serving as the precursor for prostaglandins and various other bioactive eicosanoids.
M Molnár; R Romero; F Hertelendy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  169     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1993-11-26     Completed Date:  1993-11-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  825-9     Citation Subset:  AIM; IM    
Department of Obstetrics and Gynecology, St. Louis, University School of Medicine, St. Mary's Health Center, MO.
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MeSH Terms
Analysis of Variance
Arachidonic Acid / biosynthesis*
Cells, Cultured
Cycloheximide / pharmacology
Dose-Response Relationship, Drug
Interleukin-1 / physiology*
Myometrium / cytology,  metabolism*
Oxytocin / pharmacology
Phospholipids / metabolism*
Rats, Sprague-Dawley
Time Factors
Tumor Necrosis Factor-alpha / physiology*
Uterine Contraction / drug effects
Reg. No./Substance:
0/Interleukin-1; 0/Phospholipids; 0/Tumor Necrosis Factor-alpha; 50-56-6/Oxytocin; 506-32-1/Arachidonic Acid; 66-81-9/Cycloheximide

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