Document Detail

Interleukin-1 receptor antagonist is produced by sertoli cells in vitro.
MedLine Citation:
PMID:  10746659     Owner:  NLM     Status:  MEDLINE    
The interleukin-1 (IL-1) system has been suggested to be involved in the cell-to-cell cross-talk within the testis. To identify a testicular cell source of IL-1 receptor antagonist (IL-1ra), mouse Sertoli cells were isolated, purified, cultured, and examined for IL-1ra. Our investigation revealed that Sertoli cells produce large amounts of immunoreactive IL-1ra under basal culture conditions, as examined by enzyme-linked immunosorbent assays. Its expression can be induced, showing maximum concentrations after 8 h of stimulation. Lipopolysaccharide, as well as IL-1alpha and -beta, were found to stimulate IL-1ra production in Sertoli cells. FSH is capable to induce IL-1ra production in Sertoli cells in a dose-dependent manner. Immunocytochemical staining confirmed the presence of IL-1ra in the cytoplasma of Sertoli cells. The presence of IL-1ra messenger RNA was demonstrated by RT-PCR analysis. Our results may help to better evaluate the IL-1 activity in the testis and may indicate the involvement of IL-1ra in the autocrine and paracrine regulation of testicular cell function.
D Zeyse; E Lunenfeld; M Beck; I Prinsloo; M Huleihel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  141     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-04-11     Completed Date:  2000-04-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1521-7     Citation Subset:  AIM; IM    
Department of Obstetrics and Gynecology, Soroka University Medical Center, Kiel, Germany.
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MeSH Terms
Cell Survival
Cells, Cultured
Follicle Stimulating Hormone / pharmacology
Interleukin 1 Receptor Antagonist Protein
Interleukin-1 / pharmacology
Mice, Inbred BALB C
RNA, Messenger / metabolism
Recombinant Proteins / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Sertoli Cells / metabolism*,  physiology
Sialoglycoproteins / biosynthesis*,  genetics,  metabolism
Reg. No./Substance:
0/Il1rn protein, mouse; 0/Interleukin 1 Receptor Antagonist Protein; 0/Interleukin-1; 0/RNA, Messenger; 0/Recombinant Proteins; 0/Sialoglycoproteins; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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