Document Detail


Interleukin-1 receptor and receptor antagonist gene expression after focal stroke in rats.
MedLine Citation:
PMID:  8996505     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND PURPOSE: The expression of interleukin-1 beta (IL-1 beta) is upregulated after focal brain ischemia, and previous work has demonstrated its involvement in ischemic injury. The IL-1 receptor antagonist (IL-1ra), a natural competitive antagonist of IL-1 receptors (IL-1Rs), has been demonstrated to play a role in attenuating brain ischemic injury. To hypothesize the involvement of the IL-1 system in ischemic injury, we examined other IL-1 components, including IL-1ra, IL-1RI, and IL-1RII for their mRNA expression after focal stroke. METHODS: Quantitative reverse transcription and polymerase chain reaction (RT-PCR) technique was used to examine the mRNA expression profile of IL-1ra and two IL-1R isoforms in a temporal fashion (n = 4 for each time point) after permanent occlusion of the middle cerebral artery (MCAO) in spontaneously hypertensive rats. IL-1ra and IL-1R mRNA expression was confirmed by Northern blot analysis using poly(A) RNA isolated after 2 and 12 hours of MCAO. RESULTS: Very low levels of IL-1ra mRNA were detected in sham-operated or nonischemic cortex. IL-1ra mRNA in ischemic cortex was greatly increased at 12 hours (16.5-fold increase over sham samples, P < .001) and remained elevated for up to 5 days (17.2-fold increase, P < .01) after MCAO. IL-1RI mRNA was relatively highly expressed in normal cortex and was further elevated late after ischemic injury (3.3-fold increase at day 5, P < .001). In contrast, the low basal expression of IL-1RII mRNA was remarkably elevated at 6 hours (5.3-fold increase, P < .05), reaching peak levels 12 hours (10.3-fold increase, P < .001) after MCAO. CONCLUSIONS: Differential expression of IL-1 beta, IL-1ra, IL-1RI, and IL-1RII mRNAs after focal stroke may suggest a distinct role(s) for each component of the IL-1 system in ischemic injury. The data also stress the importance of evaluating all the components of a given cytokine system (eg, agonist, receptors, and natural antagonist) after focal stroke.
Authors:
X Wang; F C Barone; N V Aiyar; G Z Feuerstein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  28     ISSN:  0039-2499     ISO Abbreviation:  Stroke     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-02-07     Completed Date:  1997-02-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  155-61; discussion 161-2     Citation Subset:  IM    
Affiliation:
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, Pa 19406, USA. xinkang-wang-1@sbphrd.com
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Blotting, Northern
Cerebral Arteries
Cerebral Cortex / immunology*,  metabolism
DNA Primers
Interleukin 1 Receptor Antagonist Protein
Ischemic Attack, Transient / immunology,  physiopathology*
Male
Polymerase Chain Reaction
RNA, Messenger / biosynthesis
Rats
Rats, Inbred SHR
Receptors, Interleukin-1 / biosynthesis*
Reference Values
Sialoglycoproteins / biosynthesis*
Time Factors
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Interleukin 1 Receptor Antagonist Protein; 0/RNA, Messenger; 0/Receptors, Interleukin-1; 0/Sialoglycoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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