| Interleukin-1 mediates endothelin-1-induced fever and prostaglandin production in the preoptic area of rats. | |
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MedLine Citation:
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PMID: 16455768 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The intracerebroventricular injection of endothelin-1 (ET-1) induces fever and increases PG levels in the cerebrospinal fluid of rats. Likewise, the injection of IL-1 into the preoptic area (POA) of the rat hypothalamus causes both fever and increased PG production. In this study, we conducted in vivo and in vitro experiments in the rat to investigate 1) the hypothalamic region involved in ET-1-induced fever and PG biosynthesis and 2) whether hypothalamic IL-1 plays a role as a mediator of the above ET-1 activities. One hundred femtomoles of ET-1 increased body temperature when injected in the POA of conscious Wistar rats; this effect was significantly counteracted by the coinjection of 600 pmol IL-1 receptor antagonist (IL-1ra). In experiments on rat hypothalamic explants, 100 nM ET-1 caused a significant increase in PGE2 production and release from the whole hypothalamus and from the isolated POA, but not from the retrochiasmatic region, in 1-h incubations. Six nanomoles of IL-1ra or 10 nM of a cell-permeable interleukin-1 converting enzyme inhibitor completely counteracted the effect of ET-1 on PGE2 release from the POA. One hundred nanomoles ET-1 also caused a significant increase in IL-1beta immunoreactivity released into the bath solution of hypothalamic explants after 1 h of incubation, although during such time ET-1 failed to modify the gene expression of IL-1beta and other pyrogenic cytokines within the hypothalamus. In conclusion, our results show that ET-1 increases IL-1 production in the POA, and this effect appears to be correlated to ET-1-induced fever in vivo, as well as to PG production in vitro. |
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Authors:
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Aline S C Fabricio; Giuseppe Tringali; Giacomo Pozzoli; Miriam C Melo; Juliana A Vercesi; Gloria E P Souza; Pierluigi Navarra |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-02 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 290 ISSN: 0363-6119 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-09 Completed Date: 2006-07-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R1515-23 Citation Subset: IM |
Affiliation:
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Institute of Pharmacology, Catholic University Medical School, Largo F. Vito 1 - 00168 Rome, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Temperature / drug effects Dinoprostone / metabolism* Endothelin-1 / pharmacology* Fever / chemically induced, metabolism* Gene Expression / drug effects Hypothalamus / drug effects, metabolism Interleukin 1 Receptor Antagonist Protein Interleukin-1 / antagonists & inhibitors, genetics, metabolism* Interleukin-6 / genetics Lipopolysaccharides / pharmacology Male Preoptic Area / drug effects, metabolism* Rats Rats, Wistar Serpins / pharmacology Sialoglycoproteins / pharmacology Viral Proteins / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Endothelin-1; 0/Interleukin 1 Receptor Antagonist Protein; 0/Interleukin-1; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Serpins; 0/Sialoglycoproteins; 0/Viral Proteins; 363-24-6/Dinoprostone; 96282-35-8/interleukin-1beta-converting enzyme inhibitor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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