Document Detail

Interleukin-1 and collagenases around loosening total hip prostheses.
MedLine Citation:
PMID:  8809439     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: The present study was carried out to assess the expression of IL-1 in periprosthetic tissue and to relate it to local collagenase levels. METHODS: Interface tissue between the implant and bone was obtained from hip revision operations and was compared to healthy joint capsular tissue. Membrane bound IL-1 alpha was stained using the avidin-biotin-peroxidase complex method and was quantified using morphometry (VIDAS image analysis system). Soluble IL-1 beta was measured by ELISA. IL-1 levels were compared to collagenase activities in the same tissue as assessed by the degradation of triple helical type I collagen monomers analyzed by SDS-PAGE/laser densitometry. RESULTS: The IL-1 alpha staining index was high in interface tissue (7.25 +/- 1.28, p < 0.01) compared to normal joint capsular tissue (0.50 +/- 0.04). Similarly, IL-1 beta levels (ng/ml; ELISA) were high in such tissue (139.0 +/- 71.9 vs 0 +/- 0, p < 0.02). Total collagenase was higher in the interface (26.3 x 10(-6) +/- 7.57 x 10(-6) IU/l, p < 0.01) than in control tissue (0.55 x 10(-6) +/- 0.44 x 10(-6) IU/l). IL-1 alpha (r = -0.04, p > 0.05) and IL-1 beta (r = 0.25, p > 0.05) did not correlate with collagenase activity in interface tissue. CONCLUSION: Both IL-1 (alpha and beta) and collagenase are up-regulated around loose THR, suggesting that they may play a role in loosening. However, the correlations between IL-1 and collagenase were not significant, indicating, that IL-1 is probably not the only cytokine involved in the regulation of collagenase activity in vivo. IL-1 may also contribute to loosening by its osteoclast activating properties.
Y T Konttinen; H Kurvinen; M Takagi; J E Michelsson; K K Eklund; L Nordsletten; L Buø; A O Aasen; S Santavirta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental rheumatology     Volume:  14     ISSN:  0392-856X     ISO Abbreviation:  Clin. Exp. Rheumatol.     Publication Date:    1996 May-Jun
Date Detail:
Created Date:  1996-12-04     Completed Date:  1996-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8308521     Medline TA:  Clin Exp Rheumatol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  255-62     Citation Subset:  IM    
Department of Anatomy, University of Helsinki, Finland.
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MeSH Terms
Aged, 80 and over
Bone and Bones / pathology
Collagenases / analysis,  metabolism*
Connective Tissue / pathology
Enzyme-Linked Immunosorbent Assay / methods
Hip Prosthesis / adverse effects*
Image Processing, Computer-Assisted / methods
Immunohistochemistry / methods
Interleukin-1 / biosynthesis*
Linear Models
Middle Aged
Osseointegration / physiology
Prosthesis Failure*
Synovial Membrane / anatomy & histology,  chemistry
Reg. No./Substance:
0/Interleukin-1; EC 3.4.24.-/Collagenases

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