Document Detail

Interleukin 1 can replace monocytes for the specific human B-cell response to a particulate antigen.
MedLine Citation:
PMID:  6610477     Owner:  NLM     Status:  MEDLINE    
It is shown that the anti-trinitrophenyl (TNP) response of human B cells to trinitrophenyl polyacrylamide beads (TNP-PAA) is monocyte dependent. This response is abolished by extensive adherent cell depletion and restored by the addition of monocytes. The optimal response is obtained with 3% monocytes, higher numbers being suppressive. Supernatants from muramyl dipeptide (MDP)-activated monocytes can restore the response of monocyte-depleted preparations even when cells are cultured at suboptimal concentration. A partially purified preparation of interleukin (IL-1) has a comparable restorative ability. The following arguments suggest that monocytes do not function as antigen-presenting cells for this particulate antigen: (i) antigen-pulsed monocytes induce neither an anti-TNP response nor a specific T-cell proliferative response; (ii) allogeneic monocytes function as well as autologous monocytes to restore the response of nonadherent cells; (iii) HLA-DR-negative cells from the human leukemia cell line K562 can replace monocytes for this response. Monocyte supernatants do not replace T cells for the response of B-enriched lymphocytes, showing that T cells are directly involved in B-cell activation.
A Vazquez; J F Balavoine; J F Delfraissy; H Wakasugi; P Galanaud
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular immunology     Volume:  86     ISSN:  0008-8749     ISO Abbreviation:  Cell. Immunol.     Publication Date:  1984 Jul 
Date Detail:
Created Date:  1984-08-20     Completed Date:  1984-08-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  287-98     Citation Subset:  IM    
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MeSH Terms
Acrylic Resins / immunology*
Antibody Formation*
Antigens / immunology*
B-Lymphocytes / cytology,  immunology*
Cell Line
Cell Separation
Cytotoxicity, Immunologic
Interleukin-1 / immunology*
Leukemia, Myeloid, Acute
Lymphocyte Activation
Monocytes / immunology*
T-Lymphocytes / immunology
Reg. No./Substance:
0/Acrylic Resins; 0/Antigens; 0/Interleukin-1; 0/trinitrophenyl polyacrylamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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