Document Detail

Interleukin-1 beta switches electrophysiological states of synovial fibroblasts.
MedLine Citation:
PMID:  9374828     Owner:  NLM     Status:  MEDLINE    
The role of electro-physiological events in signal transduction of interleukin-1 beta (IL-1 beta) was investigated in rabbit synovial fibroblasts using the perforated-patch method. Aggregated synovial fibroblasts using the perforated-patch method. Aggregated synovial fibroblasts occurred in two different electrophysiological states having membrane potentials (Vm) of -63 +/- 4 (n = 71) and -27 +/- 10 mV (n = 55) (high and low Vm, respectively). IL-1 beta affected the cells with high Vm; it switched the state of the cell from high to low Vm. This effect was strongly dependent on the external potential applied to the cell membrane. Low Vm (-30 mV) alone without IL-1 beta did not switch the state of the cells. Thus a synergistic effect involving the cytokine and cell Vm in switching the electrophysiological state of the cell was shown, indicating that electrophysiological changes are involved in signal transduction. Gap junctions between aggregated cells were necessary for the cells to have a high Vm and to respond to IL-1 beta. Gap junction resistance between adjacent cells was estimated as 300 +/- 100 M omega. Our findings suggest that the electrophysiological behavior of synovial fibroblasts is tightly connected to a signaling or intracellular mediator system that is triggered by IL-1 beta.
O V Kolomytkin; A A Marino; K K Sadasivan; R E Wolf; J A Albright
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  273     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-16     Completed Date:  1997-12-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R1822-8     Citation Subset:  IM    
Department of Orthopaedic Surgery, Louisiana State University Medical Center, Shreveport 71130-3932, USA.
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MeSH Terms
Cells, Cultured
Electric Conductivity
Fibroblasts / cytology,  drug effects,  physiology
Interleukin-1 / pharmacology*
Membrane Potentials / drug effects
Patch-Clamp Techniques
Synovial Membrane / cytology,  drug effects,  physiology*
Time Factors
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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