| Interleukin-1 beta switches electrophysiological states of synovial fibroblasts. | |
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MedLine Citation:
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PMID: 9374828 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The role of electro-physiological events in signal transduction of interleukin-1 beta (IL-1 beta) was investigated in rabbit synovial fibroblasts using the perforated-patch method. Aggregated synovial fibroblasts using the perforated-patch method. Aggregated synovial fibroblasts occurred in two different electrophysiological states having membrane potentials (Vm) of -63 +/- 4 (n = 71) and -27 +/- 10 mV (n = 55) (high and low Vm, respectively). IL-1 beta affected the cells with high Vm; it switched the state of the cell from high to low Vm. This effect was strongly dependent on the external potential applied to the cell membrane. Low Vm (-30 mV) alone without IL-1 beta did not switch the state of the cells. Thus a synergistic effect involving the cytokine and cell Vm in switching the electrophysiological state of the cell was shown, indicating that electrophysiological changes are involved in signal transduction. Gap junctions between aggregated cells were necessary for the cells to have a high Vm and to respond to IL-1 beta. Gap junction resistance between adjacent cells was estimated as 300 +/- 100 M omega. Our findings suggest that the electrophysiological behavior of synovial fibroblasts is tightly connected to a signaling or intracellular mediator system that is triggered by IL-1 beta. |
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Authors:
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O V Kolomytkin; A A Marino; K K Sadasivan; R E Wolf; J A Albright |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 273 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1997 Nov |
Date Detail:
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Created Date: 1997-12-16 Completed Date: 1997-12-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: R1822-8 Citation Subset: IM |
Affiliation:
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Department of Orthopaedic Surgery, Louisiana State University Medical Center, Shreveport 71130-3932, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Electric Conductivity Fibroblasts / cytology, drug effects, physiology Interleukin-1 / pharmacology* Membrane Potentials / drug effects Patch-Clamp Techniques Rabbits Synovial Membrane / cytology, drug effects, physiology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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