| Interleukin-1 beta stimulates somatostatin biosynthesis in primary cultures of fetal rat brain. | |
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MedLine Citation:
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PMID: 2562827 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interleukin-1 (IL-1), a secretory product of activated macrophages and many other cell types, is an important mediator of the acute phase reaction to infection and to endotoxin administration. Previous reports that GH and TSH secretion are decreased following injection of endotoxin or IL-1 led us to test the hypothesis that IL-1 acts by releasing increased amounts of somatostatin (SS), a hypothalamic factor inhibitory of both GH and TSH release. Primary cultures of dispersed fetal rat diencephalic cells were found to contain increasing amounts of immunoreactive SS in both cells and media after addition of recombinant human IL-1 beta. This increase was detectable at 24 hours and continued for up to 6 days, the longest time interval tested. Increased content of SS peptide was accompanied by marked increases in SS mRNA. These changes were dose-related, the lowest effective dose being 10(-10) M. In contrast to the long term response, exposure of the cells to IL-1 beta for one hour had only minimal stimulating effects on somatostatin release. These data indicate that IL-1 beta is neurotrophic for the somatostatinergic neuron, an action that may be responsible at least in part, for the neuroendocrine response to infection. |
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Authors:
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D E Scarborough; S L Lee; C A Dinarello; S Reichlin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 124 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1989 Jan |
Date Detail:
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Created Date: 1989-02-07 Completed Date: 1989-02-07 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 549-51 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Tufts University School of Medicine, New England Medical Center Hospitals, Boston, MA 02111. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern Brain / embryology, metabolism* Cells, Cultured Diencephalon / embryology, metabolism Interleukin-1 / pharmacology* RNA, Messenger / biosynthesis Rats Recombinant Proteins / pharmacology Somatostatin / biosynthesis*, genetics |
| Grant Support | |
ID/Acronym/Agency:
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5 F32 AM07640-02/AM/NIADDK NIH HHS; F32 AM 07931-01/AM/NIADDK NIH HHS; RR04108/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-1; 0/RNA, Messenger; 0/Recombinant Proteins; 51110-01-1/Somatostatin |
| Comments/Corrections | |
Erratum In:
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Endocrinology 1989 Apr;124(4):2022 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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