Document Detail

Interferon treatment of Ehrlich ascites tumor cells: effects on exogenous mRNA translation and tRNA inactivation in the cell extract.
MedLine Citation:
PMID:  173882     Owner:  NLM     Status:  MEDLINE    
We reported earlier that in cell extracts that were prepared from interferon-treated Ehrlich ascites tumor cells and preincubated and passed through Sephadex G-25 (S60INT), the translation of exogenous mRNA (viral and host) was impaired and the impairment could be overcome to a large extent by adding a crude tRNA preparation from Ehrlich ascites tumor cells but not from Escherichia coli. We find now that the rate of inactivation of some tRNA's (especially those specific for leucine, lysine, and serine) but not those of many others is faster in S30INT than in corresponding extracts from control cells. This increased rate of tRNA inactivation may perhaps account for the need for added RNA to overcome at least partially the impairment of translation in S30INT. The relationship of the increased rate of tRNA inactivation to the antiviral effect of interferon is unclear. So far no significant difference has been detected in the amount of tRNA needed to overcome the impairment of encephalomyocarditis virus RNA translation in S30INT between tRNA from interferon-treated cells and tRNA from control cells. Futhermore, no difference was found in the rate of inactivation in S30INT between leucine-specific tRNA's from interferon-treated and from control cells. tRNA's specific for leucine and lysine were not inactivated (unless very slowly) during incubation under out conditions in an extract from interferon-treated (or from control) cells unless the extract had been passed through Sephadex G-25 or dialyzed. The translation fo exogenous mRNA was, however, impaired in an extract from interferon-treated cells that had not been passed through Sephadex G-25. This impairment was apparently not overcome by added tRNA.
G C Sen; S L Gupta; G E Brown; B Lebleu; M A Rebello; P Lengyel
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  17     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1975 Jan 
Date Detail:
Created Date:  1976-03-11     Completed Date:  1976-03-11     Revised Date:  2010-09-01    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  191-203     Citation Subset:  IM    
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MeSH Terms
Carcinoma, Ehrlich Tumor
Cell-Free System
Culture Techniques
Dextrans / pharmacology
Encephalomyocarditis virus / metabolism
Globins / metabolism
Interferons / pharmacology*
Mengovirus / metabolism
Protein Biosynthesis / drug effects*
RNA, Messenger / metabolism*
RNA, Neoplasm / metabolism*
RNA, Transfer / metabolism*
RNA, Viral / metabolism*
Reg. No./Substance:
0/RNA, Messenger; 0/RNA, Neoplasm; 0/RNA, Viral; 9004-22-2/Globins; 9004-54-0/Dextrans; 9008-11-1/Interferons; 9014-25-9/RNA, Transfer

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