Document Detail


Interferon gamma induction during oral tolerance reduces T-cell migration to sites of inflammation.
MedLine Citation:
PMID:  10889162     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND & AIMS: Previous data suggest that oral antigen induces interferon (IFN)-gamma production in intestinal T cells. However, oral tolerance is associated with decreased production of IFN-gamma by T cells after antigen sensitization. The aim of this study was to examine the role of IFN-gamma in oral tolerance. METHODS: Oral tolerance was examined in BALB/c mice after the adoptive transfer of T cells from chicken ovalbumin (OVA(323-339))-specific, DO11.10 x RAG-1(-/-) T-cell receptor transgenic mice. RESULTS: OVA feeding induced systemic tolerance of delayed-type hypersensitivity (DTH) and antibody responses. OVA feeding up-regulated IFN-gamma production by transgenic T cells in Peyer's patch and mesenteric lymph node but not splenic tissues. Treatment of OVA-fed mice with neutralizing monoclonal antibody to IFN-gamma prevented tolerance of DTH responses. Analysis of transgenic T-cell numbers in DTH sites by immunohistochemical staining suggested that induction of IFN-gamma by oral antigen decreased accumulation of transgenic T cells in cutaneous sites of antigen injection. IFN-gamma-deficient or wild-type DO11.10 and BALB/c mice were used to show that IFN-gamma production by donor transgenic T cells was critical for oral tolerance. CONCLUSIONS: These data suggest that the induction of IFN-gamma by oral antigen contributes to systemic tolerance by decreasing migration of T cells to peripheral sites of inflammation.
Authors:
H O Lee; S D Miller; S D Hurst; L J Tan; C J Cooper; T A Barrett
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Gastroenterology     Volume:  119     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-08-03     Completed Date:  2000-08-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  129-38     Citation Subset:  AIM; IM    
Affiliation:
Division of Gastroenterology and Hepatology, Department of Medicine, Northwestern University Medical School, Chicago, Illinois, USA.
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Adoptive Transfer
Animals
Antibodies, Monoclonal / immunology,  pharmacology
Cell Movement / physiology
Chickens
Genes, RAG-1 / genetics
Hypersensitivity, Delayed / immunology,  pathology
Immune Tolerance / drug effects,  physiology*
Inflammation / physiopathology*
Interferon-gamma / biosynthesis,  immunology,  physiology*
Leukocyte Count
Mice
Mice, Inbred BALB C
Mice, Transgenic / genetics
Mouth / immunology*
Ovalbumin / pharmacology
Receptors, Antigen, T-Cell / genetics
T-Lymphocytes / immunology,  pathology,  physiology*
Grant Support
ID/Acronym/Agency:
2R01 DK47073-06A1/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Receptors, Antigen, T-Cell; 82115-62-6/Interferon-gamma; 9006-59-1/Ovalbumin
Comments/Corrections
Comment In:
Gastroenterology. 2000 Jul;119(1):260-3   [PMID:  10889179 ]

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