| Interferon-gamma induces neuroendocrine-like differentiation of human prostate basal-epithelial cells. | |
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MedLine Citation:
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PMID: 15800938 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Prostatic neuroendocrine (NE) cells are intraglandular hybrid epithelial-neural-endocrine cells that express and secrete numerous hormones and neuropeptides, which presumably regulate growth, differentiation, and secretory activity of the prostatic epithelium. This specialized cell type appears to differentiate from a common basal/precursor/stem cell that also gives rise to the secretory epithelium. In order to elucidate mechanisms of NE-differentiation the effects of type 1 (alpha, beta) and type 2 (gamma) interferons (IFNs) on human prostate basal cells (PrECs) were evaluated. METHODS AND RESULTS: Application of alpha/beta IFN increased the expression of the cell-cycle inhibitor p21(CIP1) and inhibited DNA synthesis, while only IFN-gamma led to increased apoptosis, cell-cycle inhibitor p27(KIP1) upregulation, and differentiation of PrECs into NE-like cells. In vitro differentiated NE-like cells expressed the glycolytic enzyme neuron-specific enolase (NSE) and chromogranin A (CgA), known markers of NE-cells in vivo in the prostate. These NE-like cells also changed cytokeratin (CK) expression patterns by upregulating CK 8/18, predominantly found in terminally-differentiated secretory luminal/NE epithelial cells. CONCLUSIONS: IFN-gamma produced locally in the prostate by basal cells and, under proinflammatory conditions, by infiltrating lymphocytes could support NE cell differentiation and play a role in NE differentiation processes of tumor cells in hormone-refractory prostate cancer. |
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Authors:
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Gerold Untergasser; Eugen Plas; Gerald Pfister; Elmar Heinrich; Peter Berger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Prostate Volume: 64 ISSN: 0270-4137 ISO Abbreviation: Prostate Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-07-27 Completed Date: 2005-11-08 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8101368 Medline TA: Prostate Country: United States |
Other Details:
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Languages: eng Pagination: 419-29 Citation Subset: IM |
Copyright Information:
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Copyright 2005 Wiley-Liss, Inc. |
Affiliation:
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Institute for Biomedical Aging Research, Austrian Academy of Sciences, Innsbruck, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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metabolism,
pharmacology* Apoptosis / drug effects Biological Markers Cell Differentiation / drug effects Cell Line, Tumor Epithelial Cells / cytology Growth Inhibitors / pharmacology Humans Interferon-gamma / metabolism, pharmacology* Keratins / chemistry, metabolism Male Molecular Weight Neurosecretory Systems / cytology*, metabolism Phenotype Prostate / cytology*, metabolism Prostatic Neoplasms* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Biological Markers; 0/Growth Inhibitors; 68238-35-7/Keratins; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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