| Interferon-gamma-induced neuronal differentiation of human umbilical cord blood-derived progenitors. | |
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MedLine Citation:
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PMID: 19458627 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human umbilical cord blood (HUCB) provides a source of progenitors for cell therapy. We isolated and characterized an HUCB-derived population of progenitors (HUCBNP), differentiated toward neuronal phenotype by human neuroblastoma-conditioning medium (CM) and nerve growth factor (NGF), which have been found to confer neuroprotection toward hypoxia-mediated neuronal injury. This study investigated whether interferon-gamma (IFN-gamma) contributes to HUCBNP differentiation. IFN-gamma was detected in the CM used for the induction of differentiation of HUCBNP and a neutralizing antibody of IFN-gamma significantly inhibited either IFN-gamma or CM-induced differentiation. Transcriptome analysis of CM-differentiated HUCBNP, identified 86 genes as highly upregulated, among them 25 were IFN-induced (such as 2',5'-oligoadenylate synthetase 1 and 2, IFN-induced protein and transmembrane proteins, STAT1 (IFN-gamma-receptor signal transducer and activator of transcription) and chemokine C-X-C motif ligand 5). Treatment of HUCBNP with human recombinant IFN-gamma, inhibited cell proliferation in a dose-dependent manner. IFN-gamma (1-100 ng/ml) enhanced neuronal differentiation, expressed by neurite outgrowths and increased expression of the neuronal markers beta-tubulin III, microtubule-associated protein 2, neuronal nuclei, neurofilament M and neuronal-specific enolase. IFN-gamma additively cooperated with NGF to induce the differentiation of HUCBNP. These data indicate that IFN-gamma promotes neuronal differentiation of HUCB-derived progenitors, proposing its use in future protocols towards cell therapy. |
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Authors:
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H Arien-Zakay; S Lecht; M M Bercu; N Amariglio; G Rechavi; H Galski; P Lazarovici; A Nagler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-21 |
Journal Detail:
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Title: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K Volume: 23 ISSN: 1476-5551 ISO Abbreviation: Leukemia Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-14 Completed Date: 2009-11-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8704895 Medline TA: Leukemia Country: England |
Other Details:
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Languages: eng Pagination: 1790-800 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Experimental Therapeutics, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 91120, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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2',5'-Oligoadenylate Synthetase
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genetics,
metabolism Antineoplastic Agents / pharmacology* Blotting, Western Cell Differentiation / drug effects* Cell Proliferation / drug effects Cells, Cultured Culture Media, Conditioned / pharmacology Enzyme-Linked Immunosorbent Assay Fetal Blood / cytology, drug effects*, metabolism Gene Expression Profiling Humans Immunoenzyme Techniques Interferon-gamma, Recombinant / pharmacology* Neuroblastoma / drug therapy, metabolism, pathology Neurons / cytology, drug effects*, metabolism Oligonucleotide Array Sequence Analysis RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction STAT1 Transcription Factor / genetics, metabolism Stem Cells / drug effects*, metabolism Tumor Markers, Biological / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Culture Media, Conditioned; 0/Interferon-gamma, Recombinant; 0/RNA, Messenger; 0/STAT1 Transcription Factor; 0/STAT1 protein, human; 0/Tumor Markers, Biological; EC 2.7.7.-/2',5'-Oligoadenylate Synthetase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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