Document Detail

Interferon-gamma-dependent modulation of C3b receptors (CR1) on human peripheral blood monocytes.
MedLine Citation:
PMID:  2935577     Owner:  NLM     Status:  MEDLINE    
Cell surface expression of the C3b receptor (CR1) was transiently down regulated on purified human monocytes exposed to purified recombinant human interferon-gamma(rIFN-gamma). Receptors were quantitated by using CR1-specific monoclonal antibody by radioimmunoassay or flow cytometry and by rosette analysis with the use of C3b-coated bovine erythrocytes. The reduction of CR1 was dependent on the dose of IFN-gamma used and the time of cellular exposure. Down-regulation was transient, with maximum loss occurring after 2 to 3 days of stimulation with IFN-gamma. However, after 6 days CR1 was re-expressed at the plasma membrane. This effect was observed with monocytes isolated by either centrifugal elutriation or adherence on fibronectin-coated dishes. IFN-gamma-dependent diminution of CR1 occurred concomitant with increased expression of Fc receptors and HLA-DQ antigen and unaltered expression of the C3bi receptor (CR3). The mechanism of CR1 loss from the monocyte cell surface was not due to internalization, because total cellular levels of CR1 (plasma membrane and intracellular pool) also decreased in response to IFN-gamma. These results indicate that IFN-gamma may be involved in regulating CR1 on mononuclear phagocyte surfaces.
I Esparza; R I Fox; R D Schreiber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  136     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1986-03-07     Completed Date:  1986-03-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1360-5     Citation Subset:  AIM; IM    
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MeSH Terms
Cell Line
HLA-DQ Antigens
Histocompatibility Antigens Class II / analysis
Interferon-gamma / pharmacology*
Macrophage-1 Antigen
Membrane Proteins / metabolism
Monocytes / metabolism*
Receptors, Complement / analysis,  metabolism*
Receptors, Complement 3b
Receptors, Fc / analysis
Time Factors
Grant Support
Reg. No./Substance:
0/HLA-DQ Antigens; 0/Histocompatibility Antigens Class II; 0/Macrophage-1 Antigen; 0/Membrane Proteins; 0/Receptors, Complement; 0/Receptors, Complement 3b; 0/Receptors, Fc; 82115-62-6/Interferon-gamma

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