| Interferon-gamma associated cytokines and chemokines produced by spleen cells from Brucella-immune mice. | |
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MedLine Citation:
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PMID: 15804600 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is known that interferon (IFN)-gamma plays a critical role in protection against brucellosis. In this study we have investigated several cytokines and chemokines that are associated with IFN-gamma for potential in vitro correlates of protection. We cultured spleen cells in vitro from mice immunized orally with a live, attenuated Brucella melitensis vaccine candidate (WR201) and stimulated these cells with a lysate of B. melitensis. Differential gene expression of several cytokines and chemokines in stimulated spleen cells was analysed by real-time PCR, and secreted proteins were determined by ELISA. Immunized mice produced higher levels of both protein and gene transcripts for IFN-gamma, interleukin (IL)-2, IL-18 and MIP1-alpha. Immunized mice also had elevated gene expression levels for IL12-p40, IL23-p19, IP-10, MIG and MCP-1 when compared to normal mice. In this study we have identified new cytokines and chemokines as potential immune correlates in responses to protection in Brucella-vaccinated mice. |
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Authors:
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Chrysanthi Paranavitana; Elzbieta Zelazowska; Mina Izadjoo; David Hoover |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cytokine Volume: 30 ISSN: 1043-4666 ISO Abbreviation: Cytokine Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-04-04 Completed Date: 2005-07-26 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9005353 Medline TA: Cytokine Country: United States |
Other Details:
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Languages: eng Pagination: 86-92 Citation Subset: IM |
Affiliation:
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Department of Bacterial Diseases, Walter Reed Army Institute of Research, Bldg 503, Room 3E10, Robert Grant Avenue, Silver Spring, MD 20910, USA. chrysanthi.paranavitana@na.amedd.army.mil |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brucella Vaccine / immunology Brucella melitensis / immunology* Brucellosis / prevention & control Chemokines / genetics*, immunology, metabolism Cytokines / genetics*, immunology, metabolism Female Gene Expression Regulation Interferon-gamma / genetics*, metabolism Mice Mice, Inbred BALB C Reference Values Spleen / immunology*, metabolism Vaccination |
| Chemical | |
Reg. No./Substance:
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0/Brucella Vaccine; 0/Chemokines; 0/Cytokines; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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