Document Detail


Interference of hematocrit in the tacrolimus II microparticle enzyme immunoassay.
MedLine Citation:
PMID:  12142635     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The authors studied the effect of hematocrit, bilirubin, and alkaline phosphatase on microparticle enzyme immunoassay for tacrolimus II (MEIA II) using specimens of whole blood obtained from 33 patients undergoing cyclosporine treatment. Tacrolimus was added to these samples at a final concentration of 7.5 microg/L and 15 microg/L. Both coefficients of variation were over 20% (21% at 7.5 macrog/L of tacrolimus and 22% at 15 microg/L of tacrolimus). No correlation was found between bilirubin and tacrolimus concentrations or between alkaline phosphatase and tacrolimus concentrations. On the other hand, negative correlations were found between hematocrit values and tacrolimus concentrations (r2 = 0.47; P < 0.0001 at 7.5 microg/L tacrolimus, r2 = 0.54; P < 0.0001 at 15 microg/L tacrolimus). Negative correlations were also found between hematocrit and the tacrolimus concentration using normal human red blood cells diluted with physiological saline solution (r2 = 0.93; P < 0.0001 at 7.5 microg/L tacrolimus, r2 = 0.91; P < 0.0001 at 15 microg/L tacrolimus). The results showed that the hematocrit interferes with the MEIA II for tacrolimus, and the magnitude of the interference is clinically significant. Beyond the normal range of hematocrit values, caution should be exercised in interpreting results as one may need to compensate for the levels of tacrolimus.
Authors:
Takafumi Kuzuya; Yozo Ogura; Yoko Motegi; Natsuko Moriyama; Toshitaka Nabeshima
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Therapeutic drug monitoring     Volume:  24     ISSN:  0163-4356     ISO Abbreviation:  Ther Drug Monit     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-26     Completed Date:  2003-01-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7909660     Medline TA:  Ther Drug Monit     Country:  United States    
Other Details:
Languages:  eng     Pagination:  507-11     Citation Subset:  IM    
Affiliation:
Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University School of Medicine, Nagoya, Japan. t-kuzuya@med.nagoya-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Alkaline Phosphatase / analysis
Bilirubin / analysis
Child
False Positive Reactions
Female
Hematocrit*
Humans
Immunoenzyme Techniques / methods
Immunosuppressive Agents / blood*
Male
Middle Aged
Tacrolimus / blood*
Chemical
Reg. No./Substance:
0/Immunosuppressive Agents; 109581-93-3/Tacrolimus; 635-65-4/Bilirubin; EC 3.1.3.1/Alkaline Phosphatase
Comments/Corrections
Comment In:
Ther Drug Monit. 2003 Jun;25(3):259-60   [PMID:  12766549 ]

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