| Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans. | |
| | |
MedLine Citation:
|
PMID: 17159204 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
When grown anaerobically on a succinate+nitrate (SN) medium, Paracoccus denitrificans forms the membrane-bound, cytoplasmically oriented, chlorate-reducing nitrate reductase Nar, while the periplasmic enzyme Nap is expressed during aerobic growth on butyrate+oxygen (BO) medium. Preincubation of SN cells with chlorate produced a concentration-dependent decrease in nitrate utilization, which could be ascribed to Nar inactivation. Toluenization rendered Nar less sensitive to chlorate, but more sensitive to chlorite, suggesting that the latter compound may be the true inactivator. The Nap enzyme of BO cells was inactivated by both chlorate and chlorite at concentrations that were at least two orders of magnitude lower than those shown to affect Nar. Partial purification of Nap resulted in insensitivity to chlorate and diminished sensitivity to chlorite. Azide was specific for SN cells in protecting nitrate reductase against chlorate attack, the protective effect of nitrate being more pronounced in BO cells. The results are discussed in terms of different metabolic activation of chlorine oxoanions in both types of cells, and limited permeation of chlorite across the cell membrane. |
| | |
Authors:
|
Igor Kucera |
Related Documents
:
|
12729904 - Proteasome inhibitors induce intracellular protein aggregation and cell death by an oxy... 8023704 - Photon emission from chemically perturbed yeast cells. 15040894 - The cell cycle related apoptotic susceptibility to arsenic trioxide is associated with ... 7950404 - Oxygen metabolites from lavage and interstitial lung cells after inhalation of endotoxi... 2406514 - Role of insulin during cell cycle in a myeloid cell line. 17709794 - Buccal micronucleus cytome biomarkers may be associated with alzheimer's disease. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Microbiology (Reading, England) Volume: 152 ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 2006 Dec |
Date Detail:
|
Created Date: 2006-12-12 Completed Date: 2007-03-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9430468 Medline TA: Microbiology Country: England |
Other Details:
|
Languages: eng Pagination: 3529-34 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-62500 Brno, Czech Republic. ikucera@chemi.muni.cz |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chlorates
/
metabolism,
pharmacology* Chlorides / metabolism, pharmacology* Enzyme Inhibitors / metabolism, pharmacology* Nitrate Reductase / antagonists & inhibitors, metabolism Nitrates / metabolism* Oxidation-Reduction Paracoccus denitrificans / enzymology, metabolism* Succinic Acid / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Chlorates; 0/Chlorides; 0/Enzyme Inhibitors; 0/Nitrates; 110-15-6/Succinic Acid; 1318-59-8/chlorite; EC 1.7.99.4/Nitrate Reductase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A gene cluster involved in the biosynthesis of vanchrobactin, a chromosome-encoded siderophore produ...
Next Document: Interaction between NifL and NifA in the nitrogen-fixing Pseudomonas stutzeri A1501.