Document Detail

Interference of E2-2-mediated effect in endothelial cells by FAM96B through its limited expression of E2-2.
MedLine Citation:
PMID:  21722264     Owner:  NLM     Status:  Publisher    
The basic helix-loop-helix (bHLH) protein E2-2 is known to play a role in quiescence of endothelial cells (ECs). However, how the activity of E2-2 is controlled in the cells is still unclear. In this study, we identified FAM96B as an interaction partner of E2-2. FAM96B interfered with E2-2-mediated effects on luciferase reporter activities. Furthermore, the suppression of VEGFR2 promoter activity by E2-2 was rescued by the expression of FAM96B in a dose-dependent manner. Interestingly, FAM96B decreased the expression of ectopic and endogenous E2-2 proteins. Mutational analysis revealed that the middle region of FAM96B is required for the limited expression of E2-2 protein. When FAM96B was expressed in ECs, the EC migration, proliferation and tube formation were potentiated. Taken together, these findings suggest that FAM96B acts as a regulator of E2-2 via the control of its protein expression.
Weiwen Yang; Fumiko Itoh; Hirotoshi Ohya; Fukiko Kishimoto; Aya Tanaka; Naoko Nakano; Susumu Itoh; Mitsuyasu Kato
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-1
Journal Detail:
Title:  Cancer science     Volume:  -     ISSN:  1349-7006     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101168776     Medline TA:  Cancer Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Japanese Cancer Association.
Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan Laboratory of Cardiovascular Medicine, Tokyo University of Pharmacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan Laboratory of Biochemistry High Technology Research Center, Showa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, Japan.
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