Document Detail

Interfaces between dendritic cells, other immune cells, and nerve fibres in mouse Peyer's patches: potential sites for neuroinvasion in prion diseases.
MedLine Citation:
PMID:  15816033     Owner:  NLM     Status:  MEDLINE    
In this study, we examined where immune cells and nerve fibres are located in mouse Peyer's patches, with a view to identifying potential sites for neuroinvasion by prions. Special attention was paid to dendritic cells, viewed as candidate transporters of infectious prion. Double immunofluorescence labellings with anti-CD11c antibody and marker for other immune cells (B cells, T cells, follicular dendritic cells) were carried out and analysed by confocal microscopy on Peyer's patch cryosections. To reveal the extensive ganglionated networks of the myenteric and submucosal plexi and the sparse meshworks of nerve strands, we used antibodies directed against different neurofilament subunits or against glial fibrillary acidic protein. In the suprafollicular dome, dendritic cells connect, via their cytoplasmic extensions, enterocytes with M cells of the follicle-associated epithelium. They are also close to B and T cells. Nerve fibres are detected in the suprafollicular dome, notably in contact with dendritic cells. Similar connections between dendritic cells, T cells, and nerve fibres are seen in the interfollicular region. Germinal centres are not innervated; inside them dendritic cells establish contacts with follicular dendritic cells and with B cells. After immunolabelling of normal prion protein, dendritic cells of the suprafollicular dome are intensely positive labelled.
Valérie Defaweux; Gauthier Dorban; Caroline Demonceau; Joëlle Piret; Olivier Jolois; Olivier Thellin; Caroline Thielen; Ernst Heinen; Nadine Antoine
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microscopy research and technique     Volume:  66     ISSN:  1059-910X     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-04-12     Completed Date:  2005-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-9     Citation Subset:  IM    
Copyright Information:
Copyright 2005 Wiley-Liss, Inc.
Institute of Human Histology, Immunology Center, Faculty of Medicine, University of Liège, B-4020 Liège, Belgium.
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MeSH Terms
Antigens, CD11c / metabolism
Dendritic Cells / metabolism*
Fluorescent Antibody Technique
Frozen Sections
Mice, Inbred C57BL
Nerve Fibers / metabolism*
Peyer's Patches / metabolism*
PrPC Proteins / metabolism
Reg. No./Substance:
0/Antigens, CD11c; 0/PrPC Proteins

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