Document Detail


Interface-directed self-assembly of cell-laden microgels.
MedLine Citation:
PMID:  20358531     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-laden hydrogels show great promise for creating engineered tissues. However, a major shortcoming with these systems has been the inability to fabricate structures with controlled micrometer-scale features on a biologically relevant length scale. In this Full Paper, a rapid method is demonstrated for creating centimeter-scale, cell-laden hydrogels through the assembly of shape-controlled microgels or a liquid-air interface. Cell-laden microgels of specific shapes are randomly placed on the surface of a high-density, hydrophobic solution, induced to aggregate and then crosslinked into macroscale tissue-like structures. The resulting assemblies are cell-laden hydrogel sheets consisting of tightly packed, ordered microgel units. In addition, a hierarchical approach creates complex multigel building blocks, which are then assembled into tissues with precise spatial control over the cell distribution. The results demonstrate that forces at an air-liquid interface can be used to self-assemble spatially controllable, cocultured tissue-like structures.
Authors:
Behnam Zamanian; Mahdokht Masaeli; Jason W Nichol; Masoud Khabiry; Matthew J Hancock; Hojae Bae; Ali Khademhosseini
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Small (Weinheim an der Bergstrasse, Germany)     Volume:  6     ISSN:  1613-6829     ISO Abbreviation:  Small     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-21     Completed Date:  2010-09-10     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  101235338     Medline TA:  Small     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  937-44     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Aggregation
Cell Survival
Fibroblasts / cytology*
Hydrogels / chemical synthesis*
Mice
NIH 3T3 Cells
Tissue Engineering / methods*
Tissue Scaffolds
Grant Support
ID/Acronym/Agency:
DE019024/DE/NIDCR NIH HHS; EB007249/EB/NIBIB NIH HHS; HL092836/HL/NHLBI NIH HHS; R01 HL092836/HL/NHLBI NIH HHS; R01 HL092836-01A1/HL/NHLBI NIH HHS; R01 HL092836-02/HL/NHLBI NIH HHS; R21 EB007249/EB/NIBIB NIH HHS; R21 EB007249-01/EB/NIBIB NIH HHS; R21 EB007249-02/EB/NIBIB NIH HHS; RL1 DE019024/DE/NIDCR NIH HHS; RL1 DE019024-01/DE/NIDCR NIH HHS; RL1 DE019024-02/DE/NIDCR NIH HHS; RL1 DE019024-03/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Hydrogels
Comments/Corrections

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