Document Detail


Interesterification of phosphatidylcholine with lipases in organic media.
MedLine Citation:
PMID:  1366637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipases were investigated with respect to their ability to catalyse the incorporation of fatty acids into phosphatidylcholine (PC) by interesterification reactions. The enzymes were dried onto solid support materials and the conversions were carried out in water-saturated toluene. Three lipases (two fungal and one plant enzyme) had the desired activity; immobilized lipase from Mucor miehei (Lipozyme) was the most active enzyme. The Lipozyme-catalysed interesterification was selective for the sn-1 position of PC and during 48 h of reaction around 50% of the fatty acids in this position were replaced with heptadecanoic acid, a fatty acid which was practically absent in the original phospholipid. Due to adsorption on the support material and the competing hydrolysis reaction the total amount of PC in the reaction solution decreased to about 40% of the original amount. Higher interesterification rates were obtained with free fatty acids as acyl donors than with fatty acid esters.
Authors:
I Svensson; P Adlercreutz; B Mattiasson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  33     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-12-11     Completed Date:  1990-12-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  255-8     Citation Subset:  B    
Affiliation:
Department of Biotechnology, University of Lund, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gas
Chromatography, Thin Layer
Esterification
Fatty Acids / metabolism
Kinetics
Lipase / metabolism*
Phosphatidylcholines / metabolism*
Toluene
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Phosphatidylcholines; 108-88-3/Toluene; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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