Document Detail


Interchange reaction of disulfides and denaturation of oxytocin by copper(II)/ascorbic acid/O2 system.
MedLine Citation:
PMID:  3593355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interchange reaction of disulfides was caused by the copper(II)/ascorbic acid/O2 system. The incubation of two symmetric disulfides, L-cystinyl-bis-L-phenylalanine (PP) and L-cystinyl-bis-L-tyrosine (TT), with L-ascorbic acid and CuSO4 in potassium phosphate buffer (pH 7.2, 50 mM) resulted in the formation of an asymmetric disulfide, L-cystinyl-L-phenylalanine-L-tyrosine (PT), and the final ratio of PP:PT:TT was 1:2:1. As the reaction was inhibited by catalase and DMSO only at the initial time, hydroxyl radical generated by the copper(II)/ascorbic acid/O2 system seemed to be responsible for the initiation of the reaction. Oxytocin and insulin were denatured by this system, and catalase and DMSO similarly inhibited these denaturations. As the composition of amino acids was unchanged after the reaction, hydroxyl radical was thought to cause the cleavage and/or interchange reaction of disulfides to denature the peptides.
Authors:
H Inoue; M Hirobe
Related Documents :
22158395 - High-throughput isotopic analysis of rna microarrays to quantify microbial resource use.
17918915 - Molecular similarity based on atomic electrostatic potential.
19919045 - Iodomalonic acid as an anti-inhibitor in the resorcinol inhibited briggs-rauscher react...
1479295 - Continuous fluorescence assay for lecithin:cholesterol acyltransferase using a water-so...
5646185 - On the origin of pristane in marine organisms.
20663035 - Effect of gamma-glutamylcysteine ethylester on the levels of c-fos mrna expression, glu...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  145     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1987 May 
Date Detail:
Created Date:  1987-07-15     Completed Date:  1987-07-15     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  596-603     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Ascorbic Acid / pharmacology*
Catalase / pharmacology
Copper / pharmacology*
Disulfides
Kinetics
Oxygen / pharmacology*
Oxytocin / metabolism*
Protein Denaturation
Superoxide Dismutase / pharmacology
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Disulfides; 50-56-6/Oxytocin; 50-81-7/Ascorbic Acid; 7440-50-8/Copper; 7782-44-7/Oxygen; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Enzyme-catalysed non-oxidative decarboxylation of aromatic acids: I. Purification and spectroscopic ...
Next Document:  Cap Z(36/32) is a contaminant and the major inhibitor of actin network formation in conventional act...