| Intercalation of Single-Strand Oligonucleotides between Nucleolipid Anionic Membranes: A Neutron Diffraction Study (dagger). | |
| | |
MedLine Citation:
|
PMID: 19113804 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
This contribution presents a neutron diffraction investigation of anionic lamellar phases composed of mixtures of 1-palmitoyl, 2-oleoyl phosphatidyl-nucleosides (POPN, where N is either adenosine or uridine), and POPC (1-palmitoyl,2-oleoyl-phosphatidyl-choline). Their behavior is studied for two different mole ratios and in the presence of nucleic acids. The samples are formed by the evaporation of liposomal dispersions prepared in water or in solutions containing single-strand oligonucleotides. Previous small angle X-ray scattering (SAXS) experiments on the system POPA/polyU (polyuridylic acid, high degree of polymerization, synthetic ribonucleic acid) proved that the insertion and ordering of the biopolymer in the phospholipid lamellae were driven by molecular recognition. In the present study, we extend the previous investigation to single-strand monodisperse oligonucleotides (50-mers). Structural details of the membranes were obtained from the analysis of the neutron diffraction scattering length density profiles. The evidence of direct and specific interactions, driven by molecular recognition between the nucleic polar heads of the nucleolipid and the single-strand nucleic acid, is strengthened by the comparison with identically charged bilayers formed by POPG/POPC. These results contribute to the understanding of the parameters governing the interactions between nucleolipid membranes and oligonucleotides, providing a novel strategy for the design of lipid-based vehicles for nucleic acids. |
| | |
Authors:
|
Silvia Milani; Debora Berti; Silvia Dante; Thomas Hauss; Piero Baglioni |
Related Documents
:
|
12911034 - Perfectly complementary nucleic acid enzymes. 1406734 - Use of modified nucleotides and uracil-dna glycosylase (ung) for the control of contami... 20833214 - Advanced fluorescence microscopy methods illuminate the transfection pathway of nucleic... 16375424 - Combined dft theoretical calculation and solid-state nmr studies of al substitution and... 11762454 - Effect of carvacrol and thymol on odor emissions from livestock wastes. 3491764 - The movement of organic solutes between the retina and pigment epithelium. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2008-12-29 |
Journal Detail:
|
Title: Langmuir : the ACS journal of surfaces and colloids Volume: - ISSN: 1520-5827 ISO Abbreviation: Langmuir Publication Date: 2008 Dec |
Date Detail:
|
Created Date: 2009-2-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9882736 Medline TA: Langmuir Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Chemistry and CSGI, University of Florence, via della Lastruccia 3 - Sesto Fiorentino, 50019 Florence, Italy, Italian Institute of Technology, via Morego 30, 16163 Genova, Italy, Berlin Neutron Scattering Center BENSC, Helmholtz Centre Berlin for Materials and Energy, Berlin, Germany, and Physical Biochemistry, Department of Chemistry, Technische Universitat Darmstadt, Darmstadt, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: ACCP: economic evaluations of clinical pharmacy services: 2001-2005.
Next Document: Photoregulation of ion permeation through a polyelectrolyte multilayer membrane by manipulating the ...