| Interactive fluorophore and quencher pairs for labeling fluorescent nucleic acid hybridization probes. | |
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MedLine Citation:
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PMID: 17985254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of fluorescent nucleic acid hybridization probes that generate a fluorescence signal only when they bind to their target enables real-time monitoring of nucleic acid amplification assays. Real-time nucleic acid amplification assays markedly improves the ability to obtain qualitative and quantitative results. Furthermore, these assays can be carried out in sealed tubes, eliminating carryover contamination. Fluorescent nucleic acid hybridization probes are available in a wide range of different fluorophore and quencher pairs. Multiple hybridization probes, each designed for the detection of a different nucleic acid sequence and each labeled with a differently colored fluorophore, can be added to the same nucleic acid amplification reaction, enabling the development of high-throughput multiplex assays. In order to develop robust, highly sensitive and specific real-time nucleic acid amplification assays it is important to carefully select the fluorophore and quencher labels of hybridization probes. Selection criteria are based on the type of hybridization probe used in the assay, the number of targets to be detected, and the type of apparatus available to perform the assay. This article provides an overview of different aspects of choosing appropriate labels for the different types of fluorescent hybridization probes used with different types of spectrofluorometric thermal cyclers currently available. |
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Authors:
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Salvatore A E Marras |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-11-06 |
Journal Detail:
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Title: Molecular biotechnology Volume: 38 ISSN: 1073-6085 ISO Abbreviation: Mol. Biotechnol. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-02-13 Completed Date: 2008-05-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423533 Medline TA: Mol Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 247-55 Citation Subset: IM |
Affiliation:
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Public Health Research Institute and the Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, 225 Warren Street, Newark, NJ, 07103, USA. marrassa@umdnj.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescence Resonance Energy Transfer
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methods* Fluorescent Dyes / chemistry* Models, Biological Nucleic Acid Hybridization / methods* Nucleic Acid Probes / chemistry, genetics* |
| Grant Support | |
ID/Acronym/Agency:
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EB-000277/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Nucleic Acid Probes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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