| Interactions of regenerative, inflammatory and biomechanical signals on bone morphogenetic protein-2 in periodontal ligament cells. | |
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MedLine Citation:
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PMID: 21410703 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND OBJECTIVE: Regeneration of periodontal tissues by EMD remains a major challenge because a number of modifying factors are as yet unknown. The effects of EMD seem to be mediated, at least in part, by bone morphogenetic protein-2 (BMP-2). This in vitro study was performed to examine whether the effects of EMD on BMP-2 activity are modulated by inflammatory and/or biomechanical signals. MATERIAL AND METHODS: Periodontal ligament cells were seeded on BioFlex(®) plates and exposed to EMD under normal, inflammatory or biomechanical loading conditions for 1 and 6 d. In order to mimic proinflammatory or biomechanical loading conditions in vitro, cells were stimulated with interleukin-1β (IL-1β), which is increased at inflamed periodontal sites, and cyclic tensile strain of various magnitudes, respectively. The synthesis of BMP-2, its receptors (BMPR-1A, BMPR-1B and BMPR-2) and its inhibitors (follistatin, matrix gla protein and noggin) were analyzed using real-time RT-PCR and ELISA. RESULTS: In EMD-treated cells, BMP-2 synthesis was increased significantly at 1 d. EMD also induced the expression of all BMP receptors, and of the BMP inhibitors follistatin and noggin. In general, IL-1β and biomechanical loading neither down-regulated BMP-2 nor up-regulated BMP inhibitors in EMD-stimulated cells. However, IL-1β and biomechanical loading, when applied for a longer time period, caused a down-regulation of EMD-induced BMP receptors. CONCLUSION: EMD induces not only BMP-2, but also its receptors and inhibitors, in PDL cells. IL-1β and biomechanical forces may counteract the beneficial effects of EMD on BMP-2 activity via the down-regulation of BMP receptors. |
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Authors:
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M Nokhbehsaim; B Deschner; J Winter; C Bourauel; B Rath; A Jäger; S Jepsen; J Deschner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-03-17 |
Journal Detail:
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Title: Journal of periodontal research Volume: 46 ISSN: 1600-0765 ISO Abbreviation: J. Periodont. Res. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-04-18 Completed Date: 2011-09-22 Revised Date: 2012-06-25 |
Medline Journal Info:
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Nlm Unique ID: 0055107 Medline TA: J Periodontal Res Country: Denmark |
Other Details:
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Languages: eng Pagination: 374-81 Citation Subset: D; IM |
Copyright Information:
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© 2011 John Wiley & Sons A/S. |
Affiliation:
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Clinical Research Unit, Center of Dento-Maxillo-Facial Medicine, University of Bonn, Bonn, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biomechanics Bone Morphogenetic Protein 2 / antagonists & inhibitors, drug effects, physiology* Bone Morphogenetic Protein Receptors, Type I / drug effects Bone Morphogenetic Protein Receptors, Type II / drug effects Bone Morphogenetic Proteins / antagonists & inhibitors Calcium-Binding Proteins / pharmacology Carrier Proteins / pharmacology Cells, Cultured Dental Enamel Proteins / pharmacology* Extracellular Matrix Proteins / pharmacology Follistatin / pharmacology Humans Inflammation Interleukin-1beta / pharmacology Osteogenesis / drug effects Periodontal Ligament / cytology, enzymology* Regeneration / physiology Signal Transduction / drug effects Stress, Mechanical Time Factors |
| Chemical | |
Reg. No./Substance:
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0/BMP2 protein, human; 0/Bone Morphogenetic Protein 2; 0/Bone Morphogenetic Proteins; 0/Calcium-Binding Proteins; 0/Carrier Proteins; 0/Dental Enamel Proteins; 0/Extracellular Matrix Proteins; 0/Follistatin; 0/Interleukin-1beta; 0/enamel matrix proteins; 0/matrix Gla protein; 148294-77-3/noggin protein; EC 2.7.11.30/BMPR2 protein, human; EC 2.7.11.30/Bone Morphogenetic Protein Receptors, Type I; EC 2.7.11.30/Bone Morphogenetic Protein Receptors, Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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