| Interactions of human bone cells with diamond-like carbon polymer hybrid coatings. | |
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MedLine Citation:
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PMID: 20197124 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Diamond-like carbon (DLC) coatings produced using the plasma-accelerating filtered pulsed arc discharge (FPAD) method display excellent adherence to the substrate and improve its corrosion resistance. This article reports the interactions of human osteoblastic cells with DLC and two DLC polymer hybrid (DLC-p-h) coatings deposited on smooth, matt and rough silicon wafers by the FPAD method. The DLC-p-h materials were DLC-polytetrafluoroethylene hybrid (DLC-PTFE-h) and DLC-polydimethylsiloxane hybrid (DLC-PDMS-h) coatings. The biocompatibility of the coatings was assayed by using mesenchymal stem cells, primary osteoblasts and Saos-2 cells. Human mesenchymal stem cells proliferated when cultured on DLC and DLC-PTFE-h, but their numbers diminished on DLC-PDMS-h. In all three cell types studied, phalloidin-TRITC staining disclosed cell-type organization typical of an actin cytoskeleton on DLC and DLC-PTFE-h, but minimal and disorganized stress fibers on cells cultured on DLC-PDMS-h. The microtubular cytoskeleton was similarly disorganized on DLC-PDMS-h. Cells on DLC-PDMS-h developed a peculiar form of membrane damage, with nuclear staining by propidium iodide associated with granular calcein staining of the cytoplasm. Active caspase-3 labeling was only seen in cells cultured on DLC-PDMS-h, indicating that these cells undergo apoptosis induced by defective cell adhesion. Results suggest that DLC-PDMS-h coatings might be useful in orthopedic applications where an implant or implant-facet should be protected against bone overgrowth while DLC and DLC-PTFE-h coatings might improve osseointegration. |
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Authors:
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Alicia Calzado-Martín; Laura Saldaña; Hannu Korhonen; Antti Soininen; Teemu J Kinnari; Enrique Gómez-Barrena; Veli-Matti Tiainen; Reijo Lappalainen; Luis Munuera; Yrjö T Konttinen; Nuria Vilaboa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-01 |
Journal Detail:
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Title: Acta biomaterialia Volume: 6 ISSN: 1878-7568 ISO Abbreviation: Acta Biomater Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-06-09 Completed Date: 2010-09-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101233144 Medline TA: Acta Biomater Country: England |
Other Details:
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Languages: eng Pagination: 3325-38 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Unidad de Investigación, Hospital Universitario La Paz-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation
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drug effects Actins / metabolism Bone and Bones / cytology* Caspase 3 / metabolism Cell Communication / drug effects* Cell Movement / drug effects Cell Proliferation / drug effects Cell Shape / drug effects Cell Survival / drug effects Cells, Cultured Coated Materials, Biocompatible / pharmacology* Cytoskeleton / drug effects, metabolism Diamond / pharmacology* Enzyme Activation / drug effects Humans Mesenchymal Stem Cells / cytology*, drug effects, enzymology, ultrastructure Polymers / pharmacology* Tubulin / metabolism Water / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Coated Materials, Biocompatible; 0/Polymers; 0/Tubulin; 7732-18-5/Water; 7782-40-3/Diamond; EC 3.4.22.-/Caspase 3 |
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