Document Detail

Interactions between progesterone receptor isoforms in myometrial cells in human labour.
MedLine Citation:
PMID:  11517295     Owner:  NLM     Status:  MEDLINE    
Progesterone acts to maintain uterine quiescence during pregnancy. In contrast to many other species, no decrease in maternal serum levels of progesterone can be observed in humans before the onset of labour. Therefore, a 'functional' progesterone withdrawal in association with labour has been proposed. In humans the progesterone receptor (PR) exists in two isoforms, PR-A and PR-B. While PR-B generally mediates the effects of progesterone upon gene transcription, the role of PR-A during pregnancy, and in parturition, is unknown. In this study, term myometrium cells cultured before the onset of labour were transiently transfected with expression vectors for either PR-A or PR-B. Only those cells expressing PR-B significantly increased expression of a progesterone-sensitive reporter when stimulated with progesterone. Co-transfection of both isoforms of PR demonstrated that PR-A is a dominant repressor of transactivation in these cells. Western blot analysis showed that PR-A is present in human myometrium samples taken only after, but not before, the onset of labour. These data suggest that increased expression of PR-A in human myometrium may contribute to 'functional' progesterone withdrawal and the initiation of human labour.
D Pieber; V C Allport; F Hills; M Johnson; P R Bennett
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular human reproduction     Volume:  7     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-08-22     Completed Date:  2001-11-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  875-9     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, Karl-Franzens University Graz, Austria.
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MeSH Terms
Cells, Cultured
Cesarean Section
Labor, Obstetric / genetics,  metabolism*
Myometrium / chemistry,  cytology,  metabolism*
Protein Isoforms / genetics,  metabolism
Receptors, Progesterone / biosynthesis,  genetics,  metabolism*
Transcriptional Activation
Reg. No./Substance:
0/Protein Isoforms; 0/Receptors, Progesterone; 0/progesterone receptor A; 0/progesterone receptor B

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