Document Detail


Interactions between gluconeogenesis and fatty acid oxidation in isolated sheep hepatocytes.
MedLine Citation:
PMID:  1401366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interaction of gluconeogenesis and fatty acid oxidation in isolated sheep hepatocytes was studied. Addition of tetradecylglycidic acid, an inhibitor of carnitine palmitoyltransferase I (EC 2.3.1.21), to isolated hepatocytes inhibited gluconeogenesis from a mixture of pyruvate plus lactate and from propionate alone. Inhibition constants for tetradecylglycidic acid on gluconeogenesis were 4.77 +/- 1.00 microM and 7.25 +/- 1.52 microM, respectively, for pyruvate plus lactate and for propionate as gluconeogenic substrates. The inhibition constants were not different. At the highest substrate concentrations examined, gluconeogenesis from pyruvate plus lactate and from propionate in the presence of 10 microM tetradecylglycidic acid was 47.3 and 41.4% of their respective controls. Similar to previous observations with butyrate, caproate addition inhibited gluconeogenesis from propionate by isolated hepatocytes and was unable to prevent inhibition of gluconeogenesis induced by tetradecylglycidic acid. Carnitine palmitoyltransferase I activity was lower in mitochondria isolated from hepatocytes preincubated with insulin than in control hepatocytes. The data suggest 1) that maximum rates of gluconeogenesis in isolated sheep hepatocytes from either pyruvate plus lactate or from propionate as gluconeogenic substrates require beta-oxidation, 2) that intermediates common to the metabolism of butyrate and caproate may be involved in the inhibition of propionate conversion to glucose by isolated sheep hepatocytes, and 3) that carnitine palmitoyltransferase I activity in isolated sheep hepatocytes can be modulated by insulin treatment.
Authors:
J C Chow; B W Jesse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dairy science     Volume:  75     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-10-29     Completed Date:  1992-10-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2142-8     Citation Subset:  IM    
Affiliation:
Department of Animal Sciences, Rutgers, State University, New Brunswick, NJ 08903.
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MeSH Terms
Descriptor/Qualifier:
Animals
Butyric Acid
Butyric Acids / pharmacology
Caproates / pharmacology
Carnitine O-Palmitoyltransferase / antagonists & inhibitors,  metabolism
Epoxy Compounds / pharmacology
Fatty Acids / metabolism*,  pharmacology
Gluconeogenesis* / drug effects
Lactates / metabolism
Lactic Acid
Liver / metabolism*
Mitochondria, Liver / enzymology
Oxidation-Reduction
Propionic Acids / metabolism
Pyruvates / metabolism
Pyruvic Acid
Sheep / metabolism*
Chemical
Reg. No./Substance:
0/Butyric Acids; 0/Caproates; 0/Epoxy Compounds; 0/Fatty Acids; 0/Lactates; 0/Propionic Acids; 0/Pyruvates; 107-92-6/Butyric Acid; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 68170-97-8/2-tetradecylglycidic acid; 79-09-4/propionic acid; EC 2.3.1.21/Carnitine O-Palmitoyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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